| 陈云顺 罗友军 杨苏民 公帅 杜洪彩 邢祥.尼可地尔预处理对大鼠缺血再灌注心肌mtDNA4834bp片段缺失的影响[J].,2016,16(10):1838-1841 |
| 尼可地尔预处理对大鼠缺血再灌注心肌mtDNA4834bp片段缺失的影响 |
| Effect of Nicorandil Preconditioning on mtDNA 4834 bp Deletion ofMyocardial Ischemia-reperfusion Injury in Rats |
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| DOI： |
| 中文关键词: 尼可地尔 心肌缺血再灌注损伤 线粒体DNA 线粒体ATP 敏感性钾离子通道 超氧自由基 |
| 英文关键词: Nicorandil MIRI mtDNA mitoKATPC ROS |
| 基金项目:山东省医药卫生科技发展计划项目（2014WSB26035） |
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| 中文摘要: |
| 目的：探讨尼可地尔预处理对大鼠缺血再灌注心肌mtDNA4834 bp 缺失突变的影响。方法：体重200 g-300 g，7-9 周龄健康
雄性Sprague-Dawley(SD)大鼠50 只，随机平均分为5 组，假手术（Sham）组、心肌缺血再灌注损伤（MIRI）组、缺血预处理（IPC）组、
尼可地尔（Nic）组、尼可地尔+5 羟基葵酸钠（Nic+5-HD）组。建立心肌缺血再灌注模型后取心肌组织，提取mtDNA，应用巢式PCR
法定性检测mtDNA4834 bp 片段缺失突变。结果：MIRI 组、IPC 组、Nic 组和Nic+5-HD 组突变频率分别为100%、20%、30%和
89%。Sham组未检测到mtDNA4834 bp 缺失突变。Nic 组mtDNA4834 bp 片段缺失明显低于MIRI组和Nic+5-HD组（P＜0.05），
与IPC 组比较差异无统计学意义（P＞0.05）。结论：尼可地尔预处理可降低大鼠MIRI 后mtDNA4834 bp 片段缺失突变，改善线
粒体能量衰竭。 |
| 英文摘要: |
| Objective:To investigate the effect of nicorandil preconditi oning on mitochondiral DNA 4834 bp deletion of
myochondial ischemia-reperfusion injury in rats.Methods:Weighing 200 g-300 g, aged between 7-9 weeks fifty healthy male
Sprague-Dawley(SD) rats were randomLy allocated into 5 groups(n=10 each): group Sham, group MIRI，group IPC, group Nic and group
Nic+5-HD. The animals were sacrificed and myochondial tissues were collected, mitochondiral DNA was isolation. The mitochondrial
DNA4834 bp deletion mutation was detected by nested-PCR (nested polymers chain reaction) technique.Results:The frequence of the
mitochondrial DNA 4834 bp deletion mutation of MIRI group, IPC group, Nic group and Nic+5-HD group were 100%, 20%, 30%, 89%.
The mtDNA4834 bp mutation frequency of Nic group was significantly lower than MIRI group and Nic+5-HD group (P＜0.05), there
were no significant difference between Nic group and IPC group (P ＞0.05).Conclusion:Nicorandil precondition decrease the
mtDNA4834 bp deletion mutation of rats. |
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