| 孙丽 康群甫 周明学 张蕾 刘卫红.基于生物芯片探讨高脂饮食对动脉粥样硬化小鼠基因组DNA甲基化的影响[J].,2016,16(8):1415-1419 |
| 基于生物芯片探讨高脂饮食对动脉粥样硬化小鼠基因组DNA甲基化的影响 |
| Effects of High-fat Diet on Genome DNA Methylation in of AtherosclerosisMice Model by Biochip Technology |
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| DOI： |
| 中文关键词: 动脉粥样硬化 高脂饲料 生物芯片 DNA甲基化 ApoE-/-小鼠 |
| 英文关键词: Atherosclerosis High-fat diet Biochip technology DNA methylation ApoE-/- mice |
| 基金项目:国家自然科学基金项目（81303086）；北京市自然科学基金项目（7142037） |
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| 中文摘要: |
| 目的：采用DNA甲基化芯片技术探讨高脂饮食对ApoE-/- 小鼠动脉粥样硬化模型全基因组DNA 甲基化的影响。方法：30
只雄性ApoE-/-小鼠随机分为正常组与高脂组，每组15 只，正常组给予正常饲料喂养，高脂组给予高脂饲料喂养。16 周后，测其
血脂、血清同型半胱氨酸水平（Hcy）水平、血清DNA 甲基化与血清DNA 甲基化转移酶（DNMTs）水平；采用DNA 甲基化芯片检
测两组小鼠主动脉组织全基因组甲基化情况。结果：与正常组相比，高脂组小鼠血清CHOL、TG、LDL-C 均显著升高，HDL-C 显著
下降；血清DNA甲基化水平与血清DNA 甲基化转移酶（DNMTs）水平均显著升高。甲基化芯片结果显示：与正常组相比，高脂组
主动脉全基因组中共有875 个基因甲基化发生改变，差异具有统计学意义（P< 0.05），其中高甲基化基因数目496，占总数56.69
%；低甲基化基因数目379，占总数的43.31 %。结论：高脂饲料可升高主动脉基因组甲基化水平，降低基因组的表达，可能是
ApoE-/- 小鼠容易形成动脉粥样硬化的机制之一。 |
| 英文摘要: |
| Objective:To investigate the effects of high-fat diet on genome DNA methylation in ApoE-/-mice model of
atherosclerosis by biochip technology.Methods:30 male ApoE-/-mice were divided into normal group and high-fat group with 15 in
either group. Normal group were fed with normal diet while the high-fat group were given high fat diet.16 weeks later, the blood lipids,
and the levels of homocysteine (Hcy), DNA methylation and DNA methylation transferase (DNMTs) in serum were measured; DNA
methylation chip was used to detect the genome-wide methylation in the aorta of two groups.Results:Compared with the normal group,
CHOL, TG and LDL - C of the high-fat group of mice were significantly increased (P< 0.01), and HDL - C was decreased significantly (P
< 0.05). The levels of DNA methylation and DNA methylation transferase (DNMTs) were significantly increased as well (P < 0.05).
According to the methylation chip, a total number of 875 genes in high-fat group were changed on DNA methylation in contrast with the
normal group (P < 0.05), of which 496 were hypermethylation, 56.69 %of the total, and 379 were hypomethylation, 43.31 %of the total.Conclusion:The high-fat diet can increase the level of aortic genome methylation that inhibits the genes expression which might be one
of the mechanisms of ApoE-/- mice easily to formatherosclerosis. |
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