| 陈炬辉 程龙 李航兵 孙永鑫 张兵.H2S对心跳骤停复苏大鼠脑血流动力学及神经功能的影响[J].,2016,16(6):1012-1017 |
| H2S对心跳骤停复苏大鼠脑血流动力学及神经功能的影响 |
| Effects of H2S on Cerebral Hemodynamic Parameters and NeurologicalFunction in Rats after Cardiac Arrest and Resuscitation |
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| DOI： |
| 中文关键词: H2S 脑血流 神经功能 心跳骤停 |
| 英文关键词: H2S Cerebral hemodynamic Neurological function Cardiac arrest |
| 基金项目:国家自然科学基金面上项目（81372026） |
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| 中文摘要: |
| 目的：探讨吸入外源性H2S对心跳骤停复苏大鼠脑血流动力学及神经功能的影响。方法：选择成年雄性SD 大鼠18 只，随机
分为假手术组（sham，n=6）、窒息导致心跳骤停复苏组（CA，n=6）、心跳骤停复苏+H2S 组（H2S，n=6），观察H2S对心跳骤停复苏大
鼠脑血流的影响。选择成年雄性SD 大鼠45 只，随机分为sham 组（n=5）、CA（n=20）和H2S 组（n=20），观察H2S对心跳骤停复苏大
鼠神经功能和存活率的影响。结果：CA组与H2S组在ROSC 后MAP 和CPP 均快速上升，远高于基础值(P < 0.05)；而后两组
MAP和CPP 均缓慢下降，实验结束时，明显低于基础值(P < 0.05)。两组间比较差异无统计学意义(P > 0.05)。CA 组与H2S 组CBF
曲线在ROSC 后上升至峰值，随后逐渐下降，两组在峰值无明显差异，但H2S 组CBF 减少量显著少于CA组(P < 0.05)。而在CVR
曲线，在实验最后，CA组CVR值仍明显高于基础值(P < 0.05)，而H2S 组基本降至基础值，明显低于CA组(P < 0.05)。H2S组的14
天存活率（80%）明显高于CA组(50%，P < 0.05)。在胶带移除实验（tape removal test，TRT）所需时间上，CA组与H2S 组在第1 天、
第3 天及第14 天都存在显著差异(P < 0.05)。H2S组第14 天海马CA1 区神经元存活数(33 ± 8)明显多于CA组(20 ± 6，P<0.05)，
但仍较假手术组少(53 ± 10, P < 0.05)。结论：外源性H2S 吸入对大鼠心跳骤停复苏后MAP及CPP无明显影响。外源性H2S 通过
降低CVR，改善CBF，从而改善大鼠心跳骤停复苏后的脑循环。外源性H2S能明显提高心跳骤停复苏后大鼠的存活率，降低TRT
所需时间，同时显著增加海马CA1 区神经元的存活，改善大鼠心跳骤停复苏后神经功能转归。 |
| 英文摘要: |
| Objective:To investigate the effects of exogenous hydrogen sulfide (H2S) on cerebral hemodynamic parameters and
neurological function in rats after cardiac arrest (CA) and resuscitation.Methods:18 adult male SD rats were randomly divided into three
groups: sham group (sham, n = 6), cardiac arrest group(CA, n = 6), cardiac arrest + H2S group(H2S, n = 6). To observe the effects of H2S
on cerebral hemodynamic in rats after cardiac arrest and resuscitation. 45 adult male SD rats were randomly divided into three groups:
shamgroup(n=5), CA group (n = 20), H2S group (n = 20). To observe the effects of H2S on survival rate and neurological function in rats
after CPR.Results:There was an abrupt and transient increase in MAP and CPP after ROSC in both CA group and H2S group, which was
significantly greater than the baseline value (P < 0.05); After that, MAP and CPP decreased gradually, at the end of the experiment both
of which were significantly lower than baseline values (both P < 0.05). There were no significant differences in MAP and CPP between
the CA group and the H2S group at any point during the experiment. After ROSC, the CBF increased significantly then decreased
gradually in both CA group and H2S group, but the decrease in CBF in H2S group was significantly less than that of the CA group (P <
0.05). The CVR decreased slowly in the CA group in the end, which was still significantly higher than the baseline value (P < 0.05).
Finally the CVR in H2S group almost decreased to baseline value, which significantly lower than that of the CA group (P < 0.05). There
was a significant difference in the 14-d survival rate between the CA group（50%）and H2S（80%）group (P < 0.05). Compared with the
CA group, the TRT were significantly decreased in H2S group at day 1, day 3 and day 14 after ROSC (P < 0.05). For comparison, the
number of viable neurons in the H2S group (33 ± 8) was significantly more than in the CA group (20 ± 6, P < 0.05), but still less than
that in the sham group (P < 0.05).Conclusion:Exogenous H2S has no significant influence on MAP and CPP after ROSC during the
study. Inhalation of exogenous 80ppm H2S improves CBF by decreasing the CVR CA and resuscitation. Inhalation of exogenous H2S
improved the 14d survival rate，decreased the TRT time，increased the number of the neurons in hippocampus CA1, and improved the
neurologic outcome in rats after CA and resuscitation. |
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