| 许艳 张淑娟 常志强 张善镇 尚德思.整合分析基因表达与拷贝数变异识别癌症的驱动基因及调控子miRNAs[J].,2016,16(5):940-943 |
| 整合分析基因表达与拷贝数变异识别癌症的驱动基因及调控子miRNAs |
| Integrative Analysis of Gene Expression with Copy Number VariationIdentifies Driver Genes and Regulator-miRNAs in Cancers |
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| DOI： |
| 中文关键词: 拷贝数变异 基因表达 驱动基因 调控子miRNAs |
| 英文关键词: Copy number variations Gene expression Driver gene Regulator-miRNAs |
| 基金项目:黑龙江省教育厅科技研究项目(12541278) |
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| 中文摘要: |
| 目的：通过整合分析基因的表达与拷贝数变异（CNV）识别癌症的驱动基因及调控子miRNAs。方法：通过整合基因表达与
CNV数据，分别计算了乳腺癌、结肠癌、肺癌、肾癌、膀胱癌、头颈癌六种癌症中miRNAs 的调控得分，提出了一个识别驱动基因和
显著调控子miRNAs的方法。结果：本文研究发现，CNV区域上编码的基因相比于非CNV区域上编码的基因更倾向于受miRNAs
调控。但是，癌相关CNV 区域上的基因相比正常CNV 区域上的基因更少受miRNAs 调控。本研究识别出了EXOSC4、ZNF7、
BOP1 等原癌基因，以及miR-488、miR-27a、miR-454等在多种癌症中都起调控作用的调控子miRNAs。结论：本文的方法为癌症
研究带来了新的启发，这些具有调控扩增基因过表达作用的miRNAs 的发现，有助于我们更进一步了解癌基因表达的复杂调控
机制，进而推动癌症的诊断、治疗和预后。 |
| 英文摘要: |
| Objective:By integrating analysis of gene expression and copy number variation to identify driver genes and regulator-
miRNAs in cancers.Methods:The balancer score of miRNA in six cancer types including breast carcinoma, colon adenocarcinoma,
head and neck squamous cell carcinoma, kidney renal clear cell carcinoma, lung adenocarcinoma and bladder urothelial carcinoma were
computed by integrated gene expression and copy number variation data. A novel approach was developed to identify driver genes and
significant regulator-miRNAs.Results:Our study indicated that compared with those in non-CNVs regions, genes in CNVs regions were
more likely to be regulated by miRNA, but genes in cancer-related CNVs regions were less regulated by miRNA than genes in normal
CNVs regions. We identified a set of oncogene such as EXOSC4, ZNF7, BOP1 and miRNAs which play a role as balancer in cancer cell
such as miR-488, miR-27a, miR-454.Conclusion:Our research provides a new approach to cancer study. Identifying these miRNAs
help us further understand the complicated mechanism of regulation on gene expression, and improve the diagnosis, prognosis and therapy
in cancer. |
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