| 张媛媛 郝贺玲 逄明杰 郭菲菲 孙向荣 公衍玲 徐珞.第四脑室注射Orexin-A对大鼠饮食摄取条件性位置偏爱的影响[J].,2016,16(5):847-851 |
| 第四脑室注射Orexin-A对大鼠饮食摄取条件性位置偏爱的影响 |
| Effect of Fourth ICV SB334867 on Food-conditioned Place Preference inRats |
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| DOI： |
| 中文关键词: Orexin-A 第四脑室 高脂肪饮食 条件性位置偏爱 |
| 英文关键词: Orexin-A The fourth ventricle High-fat food Conditioned place preference |
| 基金项目:国家自然科学基金项目(81470815，31071014，81100260，81270460，81300281)；
山东省优秀中青年科学家科研奖励基金项目（BS2014YY009）；青岛市科技局项目（13-1-4-170-jch；14-2-3-3-nsh） |
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| 中文摘要: |
| 目的：探讨第四脑室注射orexin-A(OXA)对大鼠饮食摄取条件性位置偏爱的影响。方法：将30 只大鼠随机分成3 组，即对照
组，低剂量组和高剂量组，第四脑室分别注射生理盐水（NS）、orexin-A 或orexin-A 受体拮抗剂SB334867，观察大鼠按压杠杆获取
蔗糖的次数和最高频率的变化。再选择30 只大鼠，第四脑室注射orexin-A 和SB334867，观察大鼠对高脂饮食(HF)食物的摄入量。
另选取30 只大鼠第四脑室注射orexin-A 或SB334867，将大鼠置于条件位置偏爱箱来检测大鼠对HF条件性位置偏爱的变化。结
果：与对照组相比，24小时禁食大鼠，第四脑室注射orexin-A，可显著增加大鼠按压杠杆获取蔗糖的次数和最高频率(P<0.05)。而
SB334867 可显著降低大鼠按压杠杆获取蔗糖次数以及最大频率(P<0.05)。第四脑室注射orexin-A，可使大鼠HF摄入量显著增加
（P<0.05），第四脑室注射SB334867，不影响大鼠HF摄入量，但会抑制普通饮食的摄入（P<0.05）。第四脑室注射orexin-A 能增强
对HF饮食位置偏爱性的表达，注射SB334867 后会显著抑制大鼠对HF 饮食位置偏爱性的表达(P<0.05)。结论：第四脑室注射
Orexin-A 可影响大鼠摄食行为，增加高脂饮食的摄入量，增强对HF饮食位置偏爱性的表达。 |
| 英文摘要: |
| Objective:To investigate the effects of the fourth ventricle injection of orexin-A (OXA) on food-conditioned place
preference in rats.Methods:30 SD male rats were randomly divided into three groups: the control group, low-dose group and high-dose
group. The fourth ventricle were injected with saline (NS), orexin-A or orexin-A receptor antagonist SB334867, observed changes of sucrose
lever presses and breakpoint of rats. Then selecting 15 rats with fourth ventricle injection orexin-A or SB334867 was observed the
effects on HF food intake during daily 1-h tests. At last, 30 rats with fourth ventricle injection orexin-A or SB334867, the rats were placed
in conditioned place preference box to detect expression of the place preference of rats with HF food.Results:Compared with the control
group, after 24 hours of fasting, The sucrose lever presses and breakpoint of rats were significantly increased through fourth ventricle injection
of orexin-A (P<0.05). But SB334867 significantly reduced sucrose lever presses and breakpoint (P<0.05). The HF intake of rats
was significantly increased with fourth ventricle injection of orexin-A (P<0.05), while SB334867 did not affect the HF intake, however
SB334867 would inhibit chow intake (P<0.05). The expression of HF food-conditioned place preference of rats was enhanced through
fourth ventricle injection of orexin-A, while SB334867 inhibited this effect(P<0.05).Conclusion:The Fourth ventricle injection of Orexin-
A may affect feeding behavior in rats, increase the intake of HF food, and enhance the expression of HF food-conditioned place preference. |
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