| 闫东霞 赵越 李耕慧 张丽丽.改良的乳鼠原代心房肌细胞的培养及鉴定方法[J].,2016,16(3):442-444 |
| 改良的乳鼠原代心房肌细胞的培养及鉴定方法 |
| Improved Primary Culture and Identification of Atrial Myocytesin Neonatal Rat |
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| DOI： |
| 中文关键词: 乳鼠 细胞培养 心房肌细胞 免疫细胞化学染色 |
| 英文关键词: Neonatal rat Cell culture Atrial myocytes Immunocytochemistry |
| 基金项目:哈尔滨市科技创新人才研究专项资金项目（2011RFQYS068） |
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| 中文摘要: |
| 目的：主要探讨原代心房肌细胞的培养及鉴定方法，为进一步研究心房颤动的重构机制及治疗方法奠定基础。方法：选取
1-3 d的SD乳鼠40 只，雌雄不限，分离心房、心室肌，胰酶联合EDTA充分消化心房肌细胞，利用心房肌与成纤维细胞的差速贴
壁及细胞传代方法纯化心房肌细胞，免疫细胞化学染色鉴定心房肌细胞。结果：心房肌细胞培养至第3 天，可见心房肌细胞覆盖
率高达90%，并出现波动性，免疫细胞化学染色可见90%的心房肌细胞肌经琢- 肌动蛋白抗体染色阳性。结论：经酶化学消化法可
成功培养出原代心房肌细胞，是一种较好的培养及鉴定乳鼠心房肌细胞的方法。 |
| 英文摘要: |
| Objective:To investigate the culture and identification of major primary atrial myocytes, which can be used to study
the remodelling of atrium or relative future research.Methods:Atrial and ventricular muscle is isolated from 40 Sprague Dawley rats(1-3
days old, male or female), trypsin EDTA is added to digest fully, then atrial myocytes were purified with differential adhesion method and
passage and identified with immunocytochemistry.Results:Atrial myocytes coverage are up to 90% after 72 hours primary culture, and
show the feature of volatility, 90% of myocytes of atrium can be authenticated by alpha-actin antibody staining with immunocytochemistry.Conclusion:Atrial myocytes with high purity and vigor can be separated by enzyme digestion, it is a better method of cultivation and identification
of atrial myocytes of neonatal rat. |
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