| Objective:The separation and purification of pepper leaf flavonoids were studied with the method of macroporous
resin, at the same time using the response surface method is optimized to get the best purification process.Methods:The extraction of
pepper leaf flavonoids were studied with the use of hot reflux method, the rates of adsorption and desorption as examining index, examine
six different types of macroporous resin (HPD100, HPD450, HPD600, HPD826, D101, AB-8) adsorption and desorption ability of hot
pepper leaf flavonoids, determine the optimal resin. The major factors which had effects on static and dynamic adsorption and desorption
of macroporous resin were investigated.Results:Through the analysis of adsorption separation performance for pepper leaf flavonoids
showed HPD600 as the best resin, the optimal process was as follows: sample concentration 10.00 mg/mL, the sample volume 10.00 mL,
elution volume of 5 BV, eluent flux 4 mL/min, eluent pH of 7, in turn, 10 %, 30%ethanol rinse with water resin column, 50%ethanol as
the eluent. The content of the purified flavonoid was 435.4 mg/g.Conclusion:This operation method was simple, the pepper leaf
flavonoids purification effect was satisfacted. |
