文章摘要
杨树萌 张浩鹏 畅建平 陶宣晨 杨拓耘 杨艳梅 张新宇.CHOP在三氧化二砷诱导SMMC-7721 凋亡中的作用[J].,2016,16(2):267-270
CHOP在三氧化二砷诱导SMMC-7721 凋亡中的作用
Role of CHOP in Arsenic Trioxide Induced SMMC-7721 Apoptosis
  
DOI:
中文关键词: 三氧化二砷  肝癌  内质网应激  CHOP
英文关键词: Arsenic trioxide  Hepatocellular carcinoma  Endoplasmic reticulumstress  CHOP
基金项目:黑龙江省自然科学基金项目(D2007-79)
作者单位
杨树萌 张浩鹏 畅建平 陶宣晨 杨拓耘 杨艳梅 张新宇 临沂市人民医院外科门诊手术室哈尔滨医科大学附属第二医院普外科哈尔滨医科大学肿瘤防治研究所 
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中文摘要:
      探讨CHOP 在三氧化二砷(Arsenic Trioxide,As2O3)诱导肝癌细胞系SMMC-7721 凋亡中的作用。方法:通过MTT法观 察不同浓度的As2O3对肝癌细胞系SMMC-7721 增殖活性的影响;通过流式细胞术检测不同浓度的As2O3作用后,SMMC-7721 细 胞的凋亡率;以Western-blot方法检测As2O3处理SMMC-7721 细胞后,内质网应激相关蛋白GRP78,CHOP 的变化。通过CHOP siRNA沉默CHOP 后,观察As2O3对SMMC-7721细胞凋亡的影响。结果:As2O3能显著抑制SMMC-7721 细胞的增殖活性,并呈 剂量依赖性诱导SMMC-7721细胞的凋亡;Western blot 结果显示三氧化二砷诱导内质网应激相关蛋白的表达。CHOP siRNA 沉 默CHOP 后能够显著抑制As2O3对SMMC-7721 细胞凋亡的诱导。结论:As2O3诱导SMMC-7721细胞凋亡可能与其诱导CHOP 表达有关。
英文摘要:
      Objective:To investigate the effect of CHOP on arsenic trioxide (As2O3) induced hepatoma carcinoma cell line SMMC-7721 apoptosis.Methods:The effect of different concentrations of As2O3 on the proliferation of SMMC-7721 was detected by MTT. The apoptosis rate of SMMC-7721 cells was detected by flow cytometry after the treatment of different concentrations of As2O3. ER stress related protein GRP78 and CHOP expression in SMMC-7721 cell was detected by Western-blot after the treatment of As2O3. The effects of As2O3 on apoptosis of SMMC-7721 cell after CHOP silenced was detected by CHOP siRNA.Results:As2O3 could significantly inhibit the proliferation of SMMC-7721 cell and it could dose-dependently induce SMMC-7721 cell apoptosis (P<0.05). Western-blot results showed the expression of endoplasmic reticulum stress related proteins GRP78 and CHOP had been changed in SMMC-7721 cell with the treatment of arsenic trioxide (P<0.05).Conclusion:The apoptosis induction of SMMC-7721 cell by As2O3 may be related to the induction of CHOP.
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