| 王燕 戴梦源 张自雄 陈始明 肖伯奎 陶泽璋.5-Aza-C 对鼻咽癌细胞端粒长度及细胞增殖的影响[J].,2015,15(36):7004-7008 |
| 5-Aza-C 对鼻咽癌细胞端粒长度及细胞增殖的影响 |
| Effect of 5-Aza-C on the Telomere Length and Cell Proliferation ofNasopharyngeal Carcinoma Cell Line |
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| DOI： |
| 中文关键词: 鼻咽癌 5-Aza-C 人端粒长度 细胞增殖活性 |
| 英文关键词: Nasopharyngeal carcinoma 5-Aza-C Telomere length Cell viability |
| 基金项目:国家自然科学基金项目（81001214, 81070766, 81372880）；湖北省自然科学基金项目（2012FFB04312） |
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| 中文摘要: |
| 目的：研究去甲基化药物5-氮杂胞嘧啶核苷（5-Azacytidine，5-Aza-C）对鼻咽癌细胞端粒长度及细胞生长增殖的影响。方法：
常规培养鼻咽癌CNE，CNE1，CNE2 及5-8F细胞系，5-Aza-C 处理鼻咽癌细胞后，甲基化测序聚合酶链反应(MSP)法检测亚端粒
区D4Z4 甲基化，端粒限制性片断检测端粒长度，CCK-8 检测细胞增殖。结果：2.5 uM浓度的5-Aza-C处理后，亚端粒区D4Z4 序
列的甲基化水平明显下降，约在15-20%之间，与处理前的甲基化水平（35-48%）有明显差异，差异均有统计学意义（P < 0.05)。在1
uM和2.5 uM浓度的5-Aza-C 处理后，四种细胞的端粒长度明显缩短，长度在2-4.5kb 之间，差异具有统计学意义(P < 0.05)。5uM
的5-Aza-C处理72 h 后，CNE，CNE1，CNE2 和5-8F 的生存率分别为51.27%，50.46%，48.85%，48.83%，10 uM 的5-azaC 处理72
h 后，CNE，CNE1，CNE2 和5-8F的生存率分别为31.64%，32.34%，30.01%，32.10%，与对照组比较差异有统计学意义（P<0.01）。结
论：5-Aza-C 能缩短端粒长度，抑制鼻咽癌细胞生长增殖活性。 |
| 英文摘要: |
| Objective:To study effect of 5-Azacytidine (5-Aza-C) treatment on telomere length and cell proliferation of
nasopharyngeal carcinoma cell line.Methods:CNE, CNE1, CNE2 and 5-8FCell lines were treated for 72 hours with 0, 1, or 2.5 uM of
the demethylating agent 5-Aza-C. Subtelomeric level methylation was evaluated with a bisulfate assay, the human telomere length was
assayed by Southern blot terminal restriction fragment analysis, the cell viability was detected using a CCK-8 assay.Results:The level of
methylation was generally high (35%-48%), but not statistically significant, treatment with 5-Aza-C significantly decreased DNA
methylation levels (15%-20%), the degree of demethylation was statistically significant (P < 0.05). The telomere lengths of the four cell
lines differed before demethylation treatment, but not remarkably. Compared with the telomere lengths of CNE and CNE1 cells (ranging
2.5-7.5 kb), those of CNE2 and 5-8F cells are highly heterogeneous (ranging 2-9 kb). The telomere lengths of cells that had been treated
with 1 uM and 2.5 uM 5-aza-dC were significantly shorter (ranging 2-4.5 kb) compared with untreated cells (P < 0.05). However, there
was no statistically significant difference between the two concentrations (P > 0.05). 5-Aza-C inhibited the growth of cell lines in a
dose-dependent manner，the survival rate was about 51.27%, 50.46%, 48.85%, 48.83%respectively in the CNE, CNE1, CNE2, and 5-8F
cells treated with 5 uM5-azaC (P<0.01) and about 31.64%, 32.34%, 30.01%, 32.10%in the cells after 72 h treated with 10 uM5-AzaC
(P<0.01).Conclusion:Our results suggest that 5-Aza-C remarkably shortened telomeres, and effectively inhibit the cell proliferation in
NPC cells. |
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