| 田甜 朱煌 蔡晓静 王洁 王天宇 牛宗镇.葛根素对极低频电磁辐射下HFSF细胞中MMP-2 与COL1A1 的影响[J].,2015,15(36):7001-7003 |
| 葛根素对极低频电磁辐射下HFSF细胞中MMP-2 与COL1A1 的影响 |
| Effect of Puerarin on MMP-2 and COL1A1 in HFSF Exposed to ELF-EMFs |
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| DOI： |
| 中文关键词: 葛根素 基质金属蛋白酶2 I型胶原 电磁辐射 巩膜成纤维细胞 |
| 英文关键词: Puerarin Matrix metalloproteinase 2 Collagen type I Electromagnetic fields Sclera fibroblast |
| 基金项目:上海市卫生局中医药科研基金项目（2014JP015A） |
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| 中文摘要: |
| 目的：研究葛根素对极低频电磁场(ELF-EMFs)诱导的人胚胎眼巩膜成纤维细胞(HFSFs)中基质金属蛋白酶-2(MMP-2)与I型
胶原(COL1Al)的影响。方法：体外培养HFSF 细胞，并将其分为对照组、辐射组、葛根素组，通过明胶酶谱法检测MMP-2的活性，
Western-Blot 检测MMP-2、COL1A1 蛋白的表达。结果：暴露于0.2 mT、50Hz 的电磁辐射系统24 h 后，HFSF 细胞(辐照组)MMP-2
酶活性较对照组增高20%，MMP-2 蛋白表达升高45%，而COL1A1的蛋白表达下降40%，差异有统计学意义(P<0.05)；而与辐射
组比较，葛根素组HFSF细胞MMP-2 酶活性下降33 %，MMP-2 蛋白表达降低44.1 %，COL1A1 蛋白升高80%，差异有统计学意
义(P<0.05)。结论：极低频电磁辐射可提高HFSF 细胞中MMP-2 的活性与蛋白表达，抑制COL1Al的合成，葛根素可在一定程度上
逆转这一作用。 |
| 英文摘要: |
| Objective:To study the effect of puerarin on matrix metalloproteinase-2 (MMP-2) and collagen typeⅠ (COL1A1) in
human fetal scleral fibroblasts (HFSFs) exposed to extremely low frequency electromagnetic fields (ELF-EMFs).Methods:HFSFs were
cultured in vitro and divided into the control group, radiation group and puerarin group. MMP-2 activity were detected by gelatin
enzymography. MMP-2 and COL1A1 protein expressions were measured by Western blot.Results:MMP-2 activity and protein
expression in radiation group increased 20% and 45%, respectively in comparison with the control group, while COL1A1 protein
expression decreased 40%(P<0.05). MMP-2 activity and protein expression in puerarin group decreased 33%and 44.1%, respectively in
comparison with radiation group, while COL1A1 protein expression increased 80% (P<0.05).Conclusion:ELF-EMFs increased the
MMP-2 activity and protein expression in HFSF cells, suppressed the synthesis of COL1A1. Puerarin could reverse the action to some
extent. |
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