| 周华 郝贺玲 逄明杰 郭菲菲 孙向荣 公衍玲 徐珞.内源性Orexin-A调控大鼠胃运动的中枢及外周机制[J].,2015,15(34):6638-6641 |
| 内源性Orexin-A调控大鼠胃运动的中枢及外周机制 |
| Effect of Endogenous Orexin-A on Gastric Motility by Central and PeripheralMechanisms in Rats |
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| DOI： |
| 中文关键词: 禁食 胃动力 Orexin-A orexin 前体 大鼠 |
| 英文关键词: Fasting Gastric motility Orexin-A Prepro-orexin Rats |
| 基金项目:：国家自然科学基金项目(81470815，31071014，81100260，81270460，81300281)；
山东省优秀中青年科学家科研奖励基金项目（BS2014YY009）；青岛市科技局项目（13-1-4-170-jch,.14-2-3-3-nsh） |
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| 中文摘要: |
| 目的：探讨内源性Orexin-A (OXA)对大鼠胃运动的中枢和外周作用机制。方法：选取成年Wistar 大鼠为研究对象，通过禁食
诱导大鼠合成内源性OXA。血浆OXA浓度采用放射免疫法测定。实验前大鼠注射OXA 受体拮抗剂SB334867，观察内源性
OXA 的作用。迷走神经切断术用来观察迷走神经的介导作用。胃排空采用分光光度法测量,消化间期胃运动通过在胃窦部植入一
应力传感器测量。Orexin 前体(PPO)在胃和下丘脑组织的表达，采用蛋白印迹确定。结果：禁食18 h 后，血浆OXA 水平和PPO 蛋
白表达显著增加(P<0.05)，在禁食36 h组达到最高水平(P<0.01)。内源性OXA 促进胃排空(P<0.05)，抑制消化间期胃蠕动(P<0.05)。
外周注射SB334867 均能阻断上述胃动力效应(P<0.05)，但对PPO表达没有影响。迷走神经切断术不能阻断内源性OXA 的介导
作用(P>0.05)。结论：禁食能诱导内源性OXA 的合成，内源性OXA能加速胃排空，同时它又抑制消化间期胃蠕动。 |
| 英文摘要: |
| Objective:To investigate the central and peripheral effects of endogenous Orexin-A (OXA) on gastric motility in rats.Methods:Selecting adult Wistar rats for the study, the synthesis of endogenous OXA was induced by fasting. Plasma OXA concentration
was measured by radioimmunoassay. Rats were pretreated with OXA receptor antagonist SB-334867 prior to measurement to observe the
effect of OXA.Vagotomy was used to observe vagus nerve-mediated effects. Gastric emptying was measured by spectrophotometric
methods. One strain gauge transducers were attached on the antrum to record interdigestive gastric motility. Preproorexin (PPO)
expression in stomach and hypothalamus were tested by Western blot.Results:After fasting 18 h, the level of plasma OXA and PPO
protein expression were significantly increased (P <0.05). The highest level of plasma OXA and PPO protein expression appeared in 36h
fasting groups (P <0.01). Endogenous OXA promoted gastric emptying (P <0.05), inhibited gastric interdigestive motility (P<0.05). As
these effects were abolished with injection of SB-334867, but no effect on PPO expression. Vagotomy surgery did not block endogenous
OXA-mediated effects (P> 0.05).Conclusion:Fasting can induce the synthesis of endogenous OXA. Endogenous OXA can accelerate
gastric emptying, but it suppressed interdigestive gastric motility. |
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