| 于翠革 李连香 朱克修 黄剑峰 李文生 王佳 陈丽宏.Chk1反义寡核苷酸联合顺铂抑制卵巢癌侵袭转移的分子机制[J].,2015,15(34):6614-6618 |
| Chk1反义寡核苷酸联合顺铂抑制卵巢癌侵袭转移的分子机制 |
| The Molecular Mechanisms Underlying CHK1-ASODN Alone or Combinedwith DDP Inhibits Human Ovarian Cancer Cells Migration and Invasion |
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| DOI： |
| 中文关键词: Chk1 反义寡核苷酸（CHK1-ASODN） 顺铂（DDP） 卵巢癌 迁移侵袭 上皮间质转化（EMT） |
| 英文关键词: CHK1-ASODN Cisplatin (DDP) Ovarian cancer Migration and invasion Epithelial-to-mesenchymal transition (EMT) |
| 基金项目:陕西省社会发展攻关项目（2012k13-02-40）；国家自然科学基金项目（81201928） |
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| 中文摘要: |
| 目的：探究Chk1 反义寡核苷酸（CHK1-ASODN）单独或联合顺铂（DDP）对卵巢癌细胞系SKOV-3 侵袭转移能力的影响，并
阐明其可能的分子机制。方法：体外培养人卵巢癌细胞系SKOV-3，CHK1-ASODN单独或联合DDP 处理48 h后，划痕实验检测
细胞迁移能力；Transwell实验检测细胞侵袭能力；显微镜下观察细胞上皮或间质表型特征；Western blot 及实时定量PCR 技术分
别检测上皮间质转化（EMT）特异性标志物（E-cadherin、N-cadherin）以及EMT 关键调控分子ZEB1 的蛋白及mRNA 的表达水平。
结果：与对照组相比较，CHK1-ASODN单独或联合DDP 均能显著抑制SKOV-3细胞的迁移及侵袭（P < 0.05）；细胞表现为间质化
表型；E-cadherin 的表达显著升高（P < 0.05），而N-cadherin 的表达则显著降低（P < 0.05）；ZEB1 的表达显著降低（P < 0.05）。结论：
CHK1-ASODN单独或联合DDP 下调ZEB1 的表达进而逆转EMT 可能是其抑制卵巢癌侵袭转移的重要机制之一。 |
| 英文摘要: |
| Objective:To explore the effects of CHK1-ASODN alone or combined with DDP on human ovarian cancer cells
migration and invasion, and investigate the molecular mechanisms underlying these effects.Methods:Human ovarian cancer cell line
SKOV-3 was cultured in vitro, and then treated with CHK1-ASODN alone or combined DDP for 48 h. The wound healing assay was
performed to detect SKOV-3 cell migration. The invasion capacity of SKOV-3 cell was determined in vitro using transwell assay. The
morphological changes of SKOV-3 cell were detected by microscope. The expression levels of E-cadherin, N-cadherin and ZEB1 protein
were detected by Western blot and real time PCR.Results:Treatment of human ovarian cancer cell line SKOV-3 with CHK1-ASODN
alone or combined with DDP resulted in significant inhibition of cell migration and invasion (P < 0.05). The results ofWestern blot and
RT-PCR showed that E-cadherin expression was upregulated and the expression of N-cadherin and ZEB1 was downregulated.Conclusion:CHK1-ASODN alone or combined with DDP can inhibit ovarian cancer cell invasion and migration, which may be through
down-regulating ZEB1expression, then reversing the EMT. |
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