| 户乃丽 徐晓雪 邹林樾 杨巍巍.利用405 nm激光激发Hoechst33342染色细胞DNA的流式细胞技术[J].,2015,15(33):6406-6409 |
| 利用405 nm激光激发Hoechst33342染色细胞DNA的流式细胞技术 |
| 405 nmLaser Exciting Hoechst33342 in Detection of Cell DNA Contentby Flow Cytometry |
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| DOI： |
| 中文关键词: 405 nm 激光 Hoechst33342 流式细胞术 细胞周期 |
| 英文关键词: 405 nmlaser Hoechst33342 Flow cytometry Cell cycle |
| 基金项目:国家自然科学基金青年基金项目(81401042) |
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| 中文摘要: |
| 目的：探讨流式细胞仪上405 nm激光激发Hoechst33342染色细胞DNA的效果及影响检测结果的因素。方法：SW480 和
A549两种细胞经Hoechst33342 染色后，流式细胞仪405 nm激光激发检测DNA 含量，利用软件计算出处于G0/G1 期、S 期和
G2/M期细胞的百分比，以PI染色法结果作为对照。结果：SW480 和A549细胞经Hoechst33342 染色后各期的细胞百分比与PI染
色法基本一致，无明显差异（P＞0.05）。结论：405 nm激光激发Hoechst33342 染色细胞DNA 结果可靠，可作为紫外检测的替代方
法。 |
| 英文摘要: |
| Objective:To observe the effect and influential factors of flow cytometry with 405 nm laser using Hoechst33342 in
detection of DNA content.Methods:SW480 and A549 cells were stained with Hoechst33342 and then excited by 405 nm laser to detect
DNA content, percentages of cells in G0/G1, S and G2/Mphases were calculated by software,results of PI staining method were used for
control.Results:The percentages of SW480 and A549 cells in each period stained with Hoechst33342 were basically same with PI
staining method（P＞0.05）Conclusion:405 nmlaser is credible in exciting Hochest33342 staining cell DNA, so it can replace ultraviolet
laser to detect DNA content. |
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