| Objective:To investigate the impact of activation to the ATP content in human unfertilized oocytes and early-stage
embryos.Methods:The unfertilized oocytes (include germinal vesicle, metaphase I, metaphase II oocytes) and early-stage embryos
(include 2-cell, 4-cell embryos) from IVF and ICSI in assisted reproduction cycles, were detected by ATP-bioluminescence assay to
analyzed the ATP content before and after activation.Results:① The ATP content of human GV, MI, MII oocytes and 2-cell,4-cell
embryos in untreated and activated group were 1.14± 0.15, 1.41± 0.14, 1.45 ± 0.12,1.61 ± 0.25, 2.05 ± 0.23 pmol, and 1.60 ± 0.17,
1.86± 0.25, 2.06± 0.38, 2.26± 0.53, 2.55± 0.46 pmol. ② The ATP content of GV and MI oocytes in activated group was significantly
higher than that in untreated group (P＜0.05).Conclusion:The ATP content of human GV, MI, MII oocytes and 2-cell, 4-cell embryos is
keep increasing; Only the ATP content of GV and MI oocytes after activation treatment were significantly higher than the untreated
group. |
