| 张晓娜 张学彦 于旸 乔虹 胥景峰.NF-kB 通路中TAK1 靶点对胃癌细胞系AGS增殖的抑制作用[J].,2015,15(28):5432-5434 |
| NF-kB 通路中TAK1 靶点对胃癌细胞系AGS增殖的抑制作用 |
| Inhibitory Effect on the Proliferation of Gastric Carcinoma Cell Line AGS byTargeting TAK1 of NF-kB Signaling Pathway |
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| DOI： |
| 中文关键词: 转化生长因子激酶1 (TAK1) NF-kB 胃癌 |
| 英文关键词: Transforming growth factor-activated kinase 1(TAK1) NF-kB Gastric cancer |
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| 中文摘要: |
| 目的：探讨NF-kB信号传导通路中转化生长因子激活激酶1(TAK1)靶点对胃癌AGS 细胞系增殖的影响。方法：利用siRNA
沉默胃癌AGS细胞系中的TAK1 基因，通过Western Blot 验证细胞中TAK1 基因的沉默情况，双项荧光素酶报告基因系统检测
TAK1 基因沉默对AGS细胞系NF-资B信号传导通路活性的影响，软琼脂克隆形成实验检测沉默TAK1 基因对胃癌AGS 细胞系
成瘤能力的影响，MTT 法检测沉默TAK1 基因对胃癌AGS细胞系增殖能力的影响。结果：与对照组Sis-Con 细胞相比，TAK1 基
因沉默组Sir-TAK1-1/Sir-TAK1-2 细胞中NF-kB信号传导通路活性明显受抑制；TAK1 基因沉默组Sir-TAK1-1/Sir-TAK1-2 细胞
软琼脂克隆形成的集落数目明显减少，差异具有统计学意义(P＜0.05)，且细胞生长的速度明显减慢。结论：TAK1 是NF-kB信号
传导通路激活的关键因子，沉默TAK1 基因可以抑制AGS胃癌细胞系的成瘤性和增殖力。 |
| 英文摘要: |
| Objective:To investigate the effect of transforming growth factor-activated kinase 1 (TAK1) of NF-kB signaling
pathway on the proliferation of AGS gastric cancer cell.Methods:TAK1 gene of AGS cells was silenced by siRNA and then confirmed
by western blot. The effect of silencing TAK1 gene on the activity of NF-kB signaling pathway, cell tumorigenicity and cell proliferation
were determined by dual-luciferase reporter gene system, soft agar clone formation assay and MTT assay, respectively.Results:The
activity of NF- kB signaling pathway in Sir-TAK1-1/Sir-TAK1-2 cells was significantly inhibited after silencing TAK1 gene when
compared with Sis-Con group cells. Moreover, the number of clone formation of Sir-TAK1-1/Sir-TAK1-2 cells was dramatically
decreased, and significant differences were found compared with Sis-Con group cells (P＜0.05). The growth of Sir-TAK1-1/Sir-TAK1-2
cells was significantly decreased.Conclusion:TAK1 gene is the key factor for activating NF-kB signaling pathway. The tumorigenicity
and proliferation of AGS gastric cancer cell lines could be inhibited by silencing TAK1 gene. |
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