| 刘阳 余海静 王男 王志超 崔永华.抗IL-6抗体治疗小鼠变应性鼻炎的作用的研究[J].,2015,15(28):5401-5404 |
| 抗IL-6抗体治疗小鼠变应性鼻炎的作用的研究 |
| Effect of Anti-IL-6 in the Treatment of Allergic Rhinitis in Mice |
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| DOI： |
| 中文关键词: 变应性鼻炎 小鼠 卵清蛋白 抗IL-6 单克隆抗体 |
| 英文关键词: Allergic rhinitis Mouse Ovalbumin Anti-IL-6 monoclonal antibody |
| 基金项目:国家自然科学基金项目（81300812） |
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| 中文摘要: |
| 目的：探讨IL-6 抗体治疗小鼠变应性鼻炎的作用。方法：实验动物分三组：PBS 组（正常对照组）、抗IL-6 抗体组（以抗IL-6 单
克隆抗体处理）、IgG 抗体对照组（以IgG对照抗体处理）。应用卵清蛋白（OVA）致敏建立小鼠变应性鼻炎模型，给予抗IL-6 单克
隆抗体干预，计量抗原激发后小鼠搔鼻数量。应用HE染色方法检测对小鼠鼻黏膜炎症影响，应用ELISA 法检测小鼠灌洗液中
IL-4、IFN-r含量。结果：治疗后抗IL-6 抗体组小鼠搔鼻症状相对于抗体对照组明显减轻（P<0.01)。与PBS 组比较，IgG 抗体对照组
小鼠鼻腔灌洗液中总炎性细胞数，淋巴细胞数及嗜酸性粒细胞数明显升高（P<0.05)；IL-6 抗体治疗后，小鼠鼻腔灌洗液中总炎性
细胞数，淋巴细胞数及嗜酸性粒细胞数明显降低（P<0.05)；IgG抗体对照组小鼠鼻腔灌洗液IL-4 含量明显升高（P<0.01)，而IFN-r
含量不变（P>0.05)；IL-6 抗体治疗后，IL-4 含量较IgG 抗体对照组降低（P<0.05)，IFN-r含量不变（P>0.05)。结论：IL-6 抗体可有效
治疗小鼠变应性鼻炎。 |
| 英文摘要: |
| Objective:To explore the effect of anti-IL-6 in the treatment of allergic rhinitis in mice.Methods:A model of allergic
rhinitis was established with Ovalbumin (OVA), and then the mice were treated with anti-IL-6 or IgG control antibody. The nasal rubs
were counted. HE staining was performed and the number of different inflammatory cells was counted in the nasal lavage. The content of
IL-4 and IFN-y were measured by ELISA.ResultsCompared to PBS group (normal control group), the nasal rubs in the IgG antibody
control group was increased significantly (P<0.01), but after the treatment of anti-IL-6, the nasal rubs was decreased (P<0.01). Compared
to PBS group, the total inflammatory cells, lymphoid cells and eosinophils showed significant difference for the IgG antibody control
group(P<0.05), but after the anti-IL-6 treatment, the number of these cells were significantly decreased (P<0.05). IFN-r was not different
among different groups (P>0.05). But after the anti-IL-6 treatment, the IL-4 production was significantly decreased (P<0.05).:Conclusion:Anti-IL-6 treatment has therapeutic effect on the allergic rhinitis of mice. |
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