| 贾立敏 贺业春 谢遵江 沈维 徐玉东 刘丽 刘颖 方传龙.透明质酸孵育树突状细胞对荷瘤小鼠免疫功能的影响[J].,2015,15(27):5252-5256 |
| 透明质酸孵育树突状细胞对荷瘤小鼠免疫功能的影响 |
| Immune Effects of Dendritic Cells Mediated by Hyaluronic Acid onImplanted B16 Melanoma Mice |
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| DOI： |
| 中文关键词: 树突状细胞 透明质酸 成熟 免疫功能 |
| 英文关键词: Dendritic cells Hyaluronic Acid Maturation Immune function |
| 基金项目:黑龙江省教育厅科学技术研究项目（10551202） |
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| 中文摘要: |
| 目的：研究透明质酸对小鼠骨髓来源树突状细胞功能的影响以及回输后荷黑色素瘤小鼠脾淋巴细胞增殖、活化和细胞因子
的变化，进而探讨透明质酸诱导的树突状细胞增强荷瘤小鼠免疫功能的机制。方法：体外细胞因子联合诱导培养小鼠骨髓细胞获
得树突状细胞（DCs），免疫磁珠分选纯化获得CD11c+树突状细胞，经不同浓度透明质酸（HA）刺激后，采用酶联免疫吸附法
（ELISA）检测培养上清液中细胞因子IL-12p70 含量。建立小鼠皮下B16 黑色素瘤模型，肿瘤局部皮下回输HA 孵育DC后检测
肿瘤大小，应用ConA 检测脾淋巴细胞增殖情况，应用MTT 法检测脾淋巴细胞杀伤活性，ELISA 法检测脾淋巴细胞分泌的
TNF-alpha和IFN-r的表达，以单纯DC回输、生理盐水注射以及正常小鼠（无瘤）组作为对照。结果：在10～100 ug/mL 范围内，HA
以剂量依赖的方式上调DCs 分泌IL-12p70。HA 孵育DC处理组肿瘤生长明显受到抑制；淋巴细胞增殖反应、杀伤活性和细胞因
子TNF-alpha和IFN-r的表达明显高于单纯DC 组和生理盐水组(P < 0.05)。结论：透明质酸可促进小鼠骨髓DC 的成熟；透明质酸孵
育的DC 通过增强荷瘤小鼠的抗肿瘤免疫功能而抑制肿瘤的生长。 |
| 英文摘要: |
| Objective:To investigate the effects of immune function of dendritic cells (DCs) mediated by Hyaluronic Acid (HA)
on the transplanted B16 melanoma in mice.Methods:Bone marrow-derived dendritic cells were induced by culture supplemented with
recombinant mouse GMSF and IL-4, and then CD11c+ cells isolated by MACS were cultured for 24 h in the presence of HA at varying
concentrations．The levels of IL-12p70 in culture supernatant of DCs were detected by ELISA．Tumor size was measured after
treatment to evaluate the reaction of the treatment. ConA methods were used to observe splenic lymphocytes proliferation. Splenic
lymphocytes were also extracted to assess the cytotoxic activity against B16 melanoma cells by MTT assay. Furthermore, both TNF-alpha
and IFN- r levels in the supernatant of culture were detected by ELISA after a 24- hour culture of spleen lymphocytes with B16
melanoma cells.Results:HA up-regulated the secretion of IL-12p70 in a dose-dependent manner at 10-100 ug /mL. Growth of the tumor
was significantly inhibited in group treated with dendritic cells mediated by Hyaluronic Acid as compared with the controls. The
lymphocytes proliferation, lymphocytes cytotoxic activity and TNF-alpha and IFN-r levels in group treated with dendritic cells mediated by
Hyaluronic Acid were significantly higher than that of the controls with statistical significance (P< 0.05).Conclusion: |
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