| 顾莹 杨舒婷 汤欣欣 尹婷 章荣 王雷雷 刘芳 王辉 顾扬 董青.不明原因复发性自然流产夫妇MTHFR C677T位点多态性分析[J].,2015,15(27):5244-5247 |
| 不明原因复发性自然流产夫妇MTHFR C677T位点多态性分析 |
| Analysis of MTHFR C677T Polymorphismin the Coupleswith Unexplained Recurrent Spontaneous Abortion |
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| DOI： |
| 中文关键词: 不明原因复发性流产 亚甲基四氢叶酸还原酶 基因多态性 |
| 英文关键词: Unexplained recurrent spontaneous abortion Methylenetetrahydrofolate reductase Polymorphism |
| 基金项目:江苏省科技支持计划-社会发展基金项目（BE2012693） |
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| 中文摘要: |
| 目的：分析不明原因复发性自然流产（URSA）夫妇与亚甲基四氢叶酸还原酶基因C677T（MTHFR C677T）位点多态性的关
联性研究。方法：采用聚合酶链式反应- 限制性片段长度多态性( PCR-RFLP)对URSA 组和对照组各50 对夫妇的外周血进行
MTHFR C677T 的位点多态性进行检测分析。结果：URSA 组MTHFR 基因677 位点的T/T、C/T+T/T 基因型的发生频率显著高于
对照组，差异具有统计学意义(P<0.05)，而对照组MTHFR 基因677 位点的C/C 基因型发生频率显著高于URSA 组（P<0.05），两
组MTHFR 基因677 位点的C/T 基因型比较无明显差异（P>0.05)。另外URSA 组等位基因T 明显高于C 的频率，且URSA 组等
位基因T 发生频率显著高于对照组，对照组等位基因C 发生频率显著高于USRA 组，差异均具有统计学意义（P<0.05）。结论：
MTHFR C677T 位点的多态性与URSA 的发生密切相关，是该病的重要遗传风险因素。 |
| 英文摘要: |
| Objective:To analyse the relationship between the couples with unexplained recurrent spontaneous abortion (URSA)
and their methylenetetrahydrofolate reductase gene C677T (MTHFR C677T) polymorphism.Methods:The MTHFR C677T
polymorphism in the peripheral blood of both URSA group (50 couples) and control group (50 couples) was detected and analyzed by
polymerase chain reaction-restricted fragment length polymorphism (PCR-RFLP).Results:The incidence of genotype T/T,C/T+T/T of
MTHFR gene locus 677 in URSA group were significantly higher than that in the control group,the differences were statistically
significant (P<0.05); the incidence of genotype C/C of MTHFR gene locus 677 in the control group was significantly higher than that in
the URSA group (P<0.05), but there was no significant difference in genotype C/T of MTHFR gene locus 677 between the two groups
(P>0.05). In addition, the incidence of allele T was significantly higher than allele C in URSA group (P<0.05), and the incidence of allele
T in URSA group was significantly higher than that in control group(P<0.05); the incidence of allele C in control group was significantly
higher than that in USRA group (P<0.05), the differences were statistically significant (P<0.05).Conclusion:MTHFR C677T
polymorphismis closely associated with the incidence of URSA, which is an important genetic risk factor of this disease. |
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