| 郑蕾 冯英明 宋扬 李红 聂娜 苏海川△.人生长分化因子15单克隆抗体制备、鉴定及药用价值初探[J].,2015,15(24):4601-4604 |
| 人生长分化因子15单克隆抗体制备、鉴定及药用价值初探 |
| Preparation, Identification and Medicinal Value of Monoclonal Antibodyagainst Growth Differentiation Factor-15 of Human |
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| DOI： |
| 中文关键词: 人GDF15 杂交瘤 单克隆抗体 |
| 英文关键词: GDF15 Hybridoma Monoclonal antibody |
| 基金项目:国家自然科学基金面上项目（81372255） |
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| 中文摘要: |
| 目的：制备稳定分泌抗人生长分化因子15（GDF15）单克隆抗体（mAb）的杂交瘤细胞系，并对其分泌的mAb 进行鉴定。方
法：根据人GDF15 氨基酸序列特征，设计合成了8 条能够免疫产生GDF15特异性抗体的抗原多肽，与VLP载体偶联后，免疫雌
性BALB/c 小鼠，利用杂交瘤技术制备鼠源抗人GDF15 的mAb，用间接ELISA 检测mAb 腹水效价。结果：获得针对7 个抗原多
肽的12 株稳定分泌抗人GDF15的杂交瘤细胞系，腹水mAb 效价可达1× 104~1× 109。结论：获得了针对不同抗原多肽的抗人
GDF15的特异性mAb，为进一步研发以GDF15 为靶点的单克隆抗体抗肿瘤药物奠定了基础。 |
| 英文摘要: |
| Objective:To establish the specific hybridoma cell lines that secreted stably monoclonal antibody (mAb) against
growth differentiation factor-15 (GDF15) from human.Methods:Eight antigen peptides which can produce GDF15 specific antibodies
were designed and coupled with VLP carrier. Then female BALB/c mice were immunized with coupled VLP carrier and mAb against
GDF15 was prepared with hybridoma cell fusion technique. Indirect enzyme-linked immunosorben assary (ELISA) was used to evaluate
the ascites titers of hybridoma cell lines.Results:Twelve hybridoma cell lines which can stable produce anti-GDF15 mAb against seven
antigen peptides were obtained. The titers of mAb in ascites were ranged from 1× 104 to 1× 109.Conclusion:The specific mAb against
different antigen peptides of GDF15 were produced, which will be significant for monoclonal antibody research of GDF15-targeted
drugs. |
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