| 杨妮 赵易 李涛 陈景荷 何丽洁 张伟 王汉民.MYH9 在肾脏纤维化中表达及作用研究[J].,2015,15(23):4410-4413 |
| MYH9 在肾脏纤维化中表达及作用研究 |
| Expression and Role of MYH9 in Renal Fibrosis |
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| DOI： |
| 中文关键词: MYH9 基因 肾脏纤维化 免疫组化 慢病毒 |
| 英文关键词: MYH9 Kidney fibrosis Immunohistochemistry Lentivirus |
| 基金项目:国家自然科学基金面上项目（81270849） |
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| 中文摘要: |
| 目的：探讨MYH9（非肌性肌球蛋白重链9）在肾脏纤维化过程中的表达变化以及这种异常表达在疾病发展过程所发挥的作
用。方法：通过免疫组化染色，Western blot以及实时PCR 检测了MYH9 在肾脏纤维化组织中表达水平的变化。同时在慢病毒介
导下构建稳定过表达MYH9 的肾脏纤维化HK-2 细胞，并通过MTT 实验、流式细胞仪检测MYH9 对HK-2 细胞增殖和细胞周期
的影响。结果：在小鼠肾脏纤维化组织中MYH9 表达水平明显高于正常小鼠肾脏组织（P＜0.05）。上调MYH9 对肾脏纤维化细胞
HK-2 的增殖水平和细胞周期都呈现出明显的促进（P＜0.05）。结论：MYH9 在肾脏纤维化过程中发挥了重要的促进作用，针对
MYH9 为靶点的研究为肾脏纤维化的治疗提供新的思路。 |
| 英文摘要: |
| Objective:To study the expression of MYH9 in kidney fibrosis, and investigate its role in the development of kidney
fibrosis.Methods:Immunohistochemical staining, Western blot and real time PCR was used to detect the changes of MYH9 expression
in kidney fibrosis. The overexpression MYH9 of renal fibrosis HK-2 cells was built stably by lentiviral-mediated transinfection. The
effects of the lentiviral-mediated MYH9 overexpression on the proliferation and cell circle of HK-2 cells were assessed by MTT assay
and flow cytometry (FCM).Results:The expression of MYH9 in mouse kidney fibrosis was significantly higher than in normal kidney
tissue of mice（P＜0.05). The overexpression of MYH9 significantly promoted the cell proliferation and cell cycle in HK-2 cells of renal
fibrosis（P＜0.05）.Conclusion:These results indicate that the MYH9 expression may be a novel and independent factor in the prognosis
of kidney fibrosis. |
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