| 李风舟 李明 蒋李懿 孟云霞 陈华.辣椒素对舌鳞癌细胞增殖和凋亡影响的初步研究[J].,2015,15(22):4221-4224 |
| 辣椒素对舌鳞癌细胞增殖和凋亡影响的初步研究 |
| Preliminary Study of the Effects of Capsaicin on Human Tongue SquamousCarcinoma cells Proliferation and Apoptosis |
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| DOI： |
| 中文关键词: 舌鳞癌 辣椒素 细胞增殖 细胞凋亡 |
| 英文关键词: Tongue Squamous Carcinoma Capsaicin Cell proliferation Apoptosis |
| 基金项目:湖南省科技厅国际合作计划基金项目（2013FJ6009）；长沙市科技计划基金项目(K1403054-31)；
广东省医学科研基金项目（A2014115）；长沙市科技局课题（K1205021-31） |
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| 中文摘要: |
| 目的：研究辣椒素对人舌鳞癌细胞增殖和存活的影响。方法：不同浓度辣椒素处理TSCCA 和Tca8113 细胞24，48 和72h，
MTS 法检测细胞增殖，Western Blot法检测细胞周期调控和细胞凋亡相关分子的表达。结果：随着辣椒素浓度的增加，p53，p21和
p27 表达上调，Bcl-2 表达未受到明显影响，Bcl-XL和Mcl-1 表达抑制，caspase7 和PARP剪切体表达上调。结论：辣椒素呈剂量依
赖和时间依赖性抑制TSCCA和Tca8113 细胞增殖，其机制可能与细胞周期和细胞凋亡相关分子表达的改变相关。 |
| 英文摘要: |
| Objective:To investigate the effects of capsaicin on proliferation and survival of human tongue squamous carcinoma
cells.Methods:TSCCA and Tca8113 cells were incubated with different concentrations of capsaicin for 24, 48 and 72h, cell
proliferation was tested by MTS and western blot was used to detect the expression the molecules which regulated cell cycle and
apoptosis.Results:With the increase of the concentration of capsaicin, the expression of p53, p21 and p27 was gradually increased, but
bcl-2 failed to be significantly upregulated. The expression of Bcl-XL and Mcl-1was inhibited, and the cleaved caspase7 and PARP was
significantly up-regulated.Conclusion:Capsaicin inhibits the proliferation of TSCCA and Tca8113 cells in a time- and dose-dependent
manner. The potential mechanismprobably related to the changed expression of the molecules which involved in cell cycle and apoptosis
regulation. |
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