| 周超 王璞 黄燚 王晗 吴越 高采平 李良平.乳酸杆菌脂磷壁酸抑制脂多糖诱导的大鼠肝脏Kupffer 细胞TLR4 通路
的激活[J].,2015,15(21):4009-4013 |
| 乳酸杆菌脂磷壁酸抑制脂多糖诱导的大鼠肝脏Kupffer 细胞TLR4 通路
的激活 |
| Lipoteichoic Acid of LactobacillusBulgaricus Inhibits theLipopolysaccharide-activated TLR4 Pathway in Rat Kupffer Cells |
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| DOI： |
| 中文关键词: Kupffer 细胞 Toll 样受体4 乳酸杆菌 脂磷壁酸 |
| 英文关键词: Kupffer cells Toll-like receptor 4 Lipoteichoic Acid |
| 基金项目:四川省卫生厅资助项目（100496） |
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| 中文摘要: |
| 目的：探讨保加利亚乳酸杆菌脂磷壁酸（Lipoteichoic Acid of ，LBG-LTA）对大鼠肝脏Kupffer细胞
Toll样受体4（Toll-like receptor 4，TLR4）信号通路的作用。方法：雄性健康Wistar大鼠10 只（2 月龄，体重250～300 g）处死后，分
离培养肝脏Kupffer 细胞；培养LBG，并提取制备LBG-LTA；Kupffer 细胞，在有或无LBG-LTA（0.1、1、10 ug/mL）预处理的情况
下，给予脂多糖（lipopolysaccharide，LPS，1 EU/mL）刺激后，Western blot 检测各孔Kupffer细胞的TLR4、TANK 结合激酶1（TANK
binding kinase-1，TBK1）及核中的核因子B（nuclear factor-kB，NF-kB）水平，酶联免疫吸附法检测各孔培养上清中的肿瘤坏死因子
alpha（tumor necrosis factor-alpha，TNF-alpha）和白介素1beta（interleukin-1beta，IL-1beta）。结果：分离的Kupffer 细胞经不同浓度LBG-LTA 预处理
后，其在LPS刺激下所表达的TLR4、TBK1、核中NF- kB的水平及生成的TNF-alpha和IL-1茁明显低于无LBG-LTA预处理情况下的
LPS 孔（P<0.05），且LBG-LTA 的作用呈浓度依赖性。结论：LBG-LTA以浓度依赖的方式抑制了LPS 诱导下大鼠Kupffer细胞
TLR4 通路的激活。 |
| 英文摘要: |
| Objective:To determine whether Lipoteichoic Acid of (LBG-LTA) inhibits Toll-like receptor
4 (TLR4) pathway in Kupffer cells isolated from rats.Methods:Ten healthy male Wistar rats, 2 months old, 250~300 g, were killed for
the isolation of Kupffer cells. Isolated Kupffer cells were treated with lipopolysaccharide (LPS, 1 EU/mL) with or without LBG-LTA
(0.1, 1 or 10 ug/mL) pretreatment. The expression of TLR4, TANK binding kinase-1 (TBK1) and activated nuclear factor-kB (NF- B) in
Kupffer cells was measured by Western blot. The production of tumor necrosis factor-alpha(TNF-alpha) and interleukin-1beta(IL-1beta) was quantified
by ELISA.Results:The LPS-induced expression of TLR4, TBK1 and activated NF- B and production of TNF-alpha and IL-1beta in isolated
Kupffer cells were significantly inhibited by LBG-LTA pretreatment in a dose-dependent manner (P<0.05).Conclusion:LBG-LTA
inhibits LPS-activated TLR4 pathway in Kupffer cells of rats in a dose-dependent manner. |
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