| 刘畅 陆慧琪 陈佳 祝丽双 李倩 韩焕兴.醛酮还原酶家族1 的C1亚型在胃癌的表达及其作用探讨[J].,2015,15(19):3621-3623 |
| 醛酮还原酶家族1 的C1亚型在胃癌的表达及其作用探讨 |
| Preliminary Discussion on the Expression and the Effect ofAldo-keto Reductase Family 1 Member C1 in Gastric Carcinoma |
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| DOI： |
| 中文关键词: AKR1C1 SGC-7901 细胞 增殖 |
| 英文关键词: AKR1C1 SGC-7901 cell Proliferation |
| 基金项目:上海市科学技术委员会项目（2014DFA33010） |
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| 中文摘要: |
| 目的：研究AKR1C1（Aldo-keto reductase family 1 member C1）在胃癌中的表达，并初步探讨其在胃癌发生和发展中的作用。
方法：采用胃癌组织芯片和免疫组化分析方法，检测60 例胃癌患者癌组织及癌旁正常组织AKR1C1 的表达；实时荧光定量PCR
和蛋白免疫印迹western blot 检测胃癌细胞株SGC-7901 AKR1C1 的表达；在胃癌细胞株SGC-7901 中转染沉默AKR1C1 的
shRNA质粒及空质粒，MTT 比色法检测各实验组细胞增殖。结果：组织芯片和免疫组化结果显示，与正常组织相比，胃癌组织中
AKR1C1 呈高表达；实时荧光定量PCR 和western blot观察可以发现胃癌细胞株SGC-7901 高表达AKR1C1 ；MTT 比色法检测
发现，转染沉默AKR1C1 的shRNA 质粒组与空质粒对照组相比，SGC-7901 细胞的增殖受到明显抑制，差异有统计学意义（P＜
0.05)。结论：AKR1C1 与癌细胞的增殖有关，可能是其参与或间接参与了癌细胞的生长周期，为胃癌的发生及细胞增值提供了新
的研究思路和方向。 |
| 英文摘要: |
| Objective:To investigate the expression ofAKR1C1 (aldo-keto reductase family 1 member C1) in gastric carcinoma
and discuss its role in gastric carcinogenesis and development.Methods:Gastric cancer tissue microarray and immunohistochemical
method were used to detect the expression of AKR1C1 in gastric carcinoma tissue microarray of 60 patients. Use the method of real-time
fluorescence quantitative PCR and protein western blot to detect the expression of AKR1C1 in gastric cancer cell lines SGC-7901.
Transfect gene silencing AKR1C1 short hairpin RNA plasmid and empty plasmid in gastric cancer cell lines SGC-7901 and use the
method of MTT colorimetric assay to detect cell proliferation.Results:Tissue microarray and immunohistochemical method showed that
the expression of AKR1C1 in the cancer tissue was higher than that in normal tissue. High expression of AKR1C1 in gastric cancer cell
lines SGC-7901 was detected by the method of real-time fluorescence quantitative PCR and protein western blot. The method of MTT
colorimetric assay found that cell proliferation was significantly inhibited in gene silencing AKR1C1 short hairpin RNA plasmid
transfection group, compared with empty plasmid and blank group. And the difference has statistical significance（P＜0.05).Conclusion:AKR1C1 has relationship with proliferation of cancer cell. The reason may be that AKR1C1 is directly or indirectly involved in the
growth cycle of cancer cell.Provide new research ideas and directions for gastric cancer proliferation occurs. |
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