| 马琳 苏靖 邱明丰 盛净 陆平.聚乙二醇化学修饰的聚乙烯亚胺衍生物的制备及其细胞毒性研究[J].,2015,15(19):3616-3620 |
| 聚乙二醇化学修饰的聚乙烯亚胺衍生物的制备及其细胞毒性研究 |
| Study on Preparation and Cytotoxicity ofPolyethylenimine Derivatives Modified by Polyethylene Glycol |
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| DOI： |
| 中文关键词: 基因输送 聚乙烯亚胺（PEI） PET 细胞毒性 |
| 英文关键词: Gene Delivery Polyethylenimine(PEI) PET Cytotoxicity |
| 基金项目:国家自然科学基金项目（81300092） |
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| 中文摘要: |
| 目的：合成聚乙二醇（PEG）化的聚乙烯亚胺衍生物（PEI-Et）基因输送载体PET 1和PET 2，并考察两个载体材料在HeLa 细
胞、MCF-7 细胞中的细胞毒性及在HeLa 细胞中的转染效率。方法：将亚乙基二氯甲酸酯与PEI 800 Da 交联制备成交联PEI衍生
物PEI-Et，进一步将PEI-Et与聚乙二醇（PEG）以不同摩尔比例（1:1，2:1）交联连接，得到PEG化的PET 1和PET 2。采用MTT 法
检测PEI-Et、PET 1、PET 2对HeLa 细胞、MCF-7 细胞的细胞毒性。检测单位质量的荧光强度测定转染效率。结果：PET 的细胞毒
性随浓度增大而增大，在同一浓度下PET的细胞毒性小于PEI 25 KDa（P<0.01）；并且与DNA 复合后，复合物细胞毒性随质量比
的增高而增大，在同一质量比下PET 的细胞毒性小于PEI 25 KDa（P<0.01），特别是PET 1。并且最佳比例时，PET 1的转染活性最
高。结论：作为非病毒基因载体，PET 1具有高的转染效率及低的细胞毒性。 |
| 英文摘要: |
| Objective:Gene delivery vectors: PET 1 and PET 2 were synthesized by chemically conjugating polyethylene glycol
(PEG) with polyethylene imine derivatives (PEI-Et). Respectively, the article investigated the cytotoxicity of the two vectors on HeLa
cells, MCF-7 cells and transfection efficiency on HeLa cells.Methods:PEI-Et was prepared by cross-linking PEI 800 Da with ethylene
biscarbamate linkages. Then PEI-Et were conjugated with polyethylene glycol (PEG) in different molar ratios (1:1, 2:1), to get the PET 1
and PET 2. MTT method was used to detect the toxicity of PEI-Et, PET 1, PET 2 on HeLa cells, MCF-7 cells. The transfection efficiency
was detected by the fluorescence intensity.Results:The cytotoxicity of PET increased with the increasing of the concentrations and
weigh ratios. Compared to PEI 25 KDa, PET cytotoxicity was significantly reduced at the same concentration and weigh ratio, especially
PET 1. And the transfection efficiency of PET 1 was the highest at the optimal weight ratio.Conclusion:As a non-viral gene carrier，
PET 1 showed high transfection efficiency and low cytotoxicity. |
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