| 陈宇亮 张亚楠 张婷 徐伟刚 李润平.GLT-1 在高压氧致中枢神经系统氧中毒发生中的作用研究[J].,2015,15(18):3422-3425 |
| GLT-1 在高压氧致中枢神经系统氧中毒发生中的作用研究 |
| Effects of Glutamate Transporter GLT-1 in Central Nervous SystemOxygenToxicity Caused by Hyperbaric Oxygen Exposure |
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| DOI： |
| 中文关键词: 高压氧 中枢神经系统氧中毒 谷氨酸 谷氨酸转运体 |
| 英文关键词: Hyperbaric oxygen Central nervous systemoxygen toxicity Glutamate GLT-1 |
| 基金项目:国家自然科学基金项目（81272179）；第二军医大学军事医学专项课题（2011JS02） |
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| 中文摘要: |
| 目的：探讨谷氨酸及其转运体在高压氧（hyperbaric oxygen，HBO）致中枢神经系统氧中毒（central nervous system oxygen
toxicity，CNS-OT）发生中的作用。方法：（1）大鼠侧脑室注射谷氨酸转运体（Glutamate Transporter-1, GLT-1）选择性激动剂
Ceftriaxone 后观察氧惊厥潜伏期。采用随机数字法将大鼠分为对照组和50 ug、100 ug以及200 ug Ceftriaxone 给药组。采用侧脑
室注射方法分别给予生理盐水和不同剂量Ceftriaxone 后，进行0.6 MPa HBO暴露，记录大鼠的惊厥潜伏期。（2）大鼠侧脑室注射
GLT-1 选择性抑制剂DHK 后观察氧惊厥潜伏期。采用随机数字法将大鼠分为对照组和5 ug、10 ug以及20 ug DHK给药组。采
用脑室注射方法分别给予生理盐水和不同剂量DHK 后，进行0.6 MPa HBO 暴露，记录大鼠的惊厥潜伏期。结果：脑室注射100
滋g Ceftriaxone 组（33 分4.2 秒4 分12.4 秒）和200 ug Ceftriaxone 组（47 分4.2 秒5 分5.2 秒）惊厥潜伏期显著延长，差异有统计
学意义（P 0.05），并存在一定量效关系。脑室注射5 ug DHK组（16 分44.4 秒± 2 分4.7 秒）、10 ug DHK组（12 分51 秒± 1 分23.3
秒）和20 ug DHK组（7 分31.1 秒± 53 秒）惊厥潜伏期显著缩短，差异有统计学意义（P 0.05），并存在一定量效关系。结论：中枢
局部给予GLT-1 激动剂可以有效延长CNS-OT 的潜伏期；中枢局部给予GLT-1 抑制剂可以有效缩短CNS-OT 的潜伏期。 |
| 英文摘要: |
| Objective:To investigate the effects of glutamate and glutamate transporter (GLT-1) in central nervous system oxygen
toxicity caused by hyperbaric oxygen exposure.Methods:1. The changes of the latency of central nervous system oxygen toxicity after
intracerebroventricularly injecting GLT-1 agonist Ceftriaxone. 24 sprague-dawley rats were randomly divided into 4 groups, each
consisting of 6 animals: the saline group (the animals were intracerebroventricularly injected 20 滋L saline ), 50 滋g Ceftriaxone group(the
animals were intracerebroventricularly injected 50 滋g Ceftriaxone), 100 滋g Ceftriaxone group(the animals were intracerebroventricularly
injected 100 滋g Ceftriaxone), 200 滋g Ceftriaxone group (the animals were intracerebroventricularly injected 200 滋g Ceftriaxone). The
latency of central nervous system oxygen toxicity was observed and recorded during the exposure of 6 ATA hyperbaric oxygen. 2. The
changes of the latency of central nervous system oxygen toxicity after intracerebroventricularly injecting GLT-1 antagonist DHK. 24
sprague-dawley rats were randomly divided into 4 groups, each consisting of 6 animals: the saline group (the animals were
intracerebroventricularly injected 20 uL saline), 5 ug DHK group(the animals were intracerebroventricularly injected 5 ug DHK), 10 ug
DHK group (the animals were intracerebroventricularly injected 10 ug DHK), and 20 ug DHK group (the animals were
intracerebroventricularly injected 20 ug DHK). The latency of central nervous system oxygen toxicity was observed and recorded during
the exposure of 6 ATA hyperbaric oxygen.Results:The latency of central nervous system oxygen toxicity of 100 ug Ceftriaxone group
(33 min 4.2 sec 4 min 12.4 sec) and 200 ug Ceftriaxone group(47 min 4.2 sec 5 min 5.2 sec) were significantly longer than that of saline
group and there was dose-response relationship between the two groups. The latency of central nervous system oxygen toxicity of 5 ug
DHK group(16 min 44.4 sec 2 min 4.7sec), 10 ug DHK group(12 min 51 sec 1min 23.3 sec) and 20 ug DHK group(7 min 31.1 sec 53
sec) were significantly longer than that of the saline group and there was dose-response relationship among the three groups.Conclusion:The latency of central nervous system oxygen toxicity could be prolonged after intracerebroventricularly injecting GLT-1 agonist
Ceftriaxone or shortened after intracerebroventricularly injecting GLT-1 antagonist DHK. |
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