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目的：探讨NF-kB 和TGF-beta1 在超声乳化术治疗的白内障患者结膜上皮细胞中的表达及其意义。方法：选取我院眼科收治的白内障患者24 例，共24 眼，且均接受超声乳化白内障摘除术和后房型人工晶状体植入术治疗。在治疗前和治疗后15天、30 天，采用免疫组织化学法检测患者结膜上皮细胞中NF-kB 和TGF-beta1 的表达，并进行泪液分泌试验、荧光素染色、泪膜破裂时间和泪河高度测量四项检查。结果：NF-资B 和TGF-茁1 呈现先增强后减弱的趋势，与术前相比，术后15 天患者NF-kB 和TGF-beta1 染色程度显著增强，差异具有统计学意义，(P<0.05)；与术后15 天比较，术后30 天NF-kB 和TGF-beta1 染色程度减弱，差异具有统计学意义，(P<0.05)；与术前相比，术后15 天泪膜破裂时间较术前明显缩短，荧光素染色明显增强；与术前相比，术后30 天四项检查结果无差异，P＞0.05。结论：超声乳化手术可提高白内障患者结膜上皮细胞中NF-kB 和TGF-beta1的表达，与术后眼膜的炎症反应有关。

英文摘要:

Objective:To investigate the expression of NF-kB and TGF-beta1 in phacoemulsification for treatment of conjunctival epithelial cells in cataract patients.Methods:24 cases of cataract patients were selected, a total of 24 eyes, were treated with phacoemulsificationand posterior chamber intraocular lens implantation. Detected NF-kB and TGF-beta 1 expression by immunohistochemistry in conjunctiva epithelial cells, and check Schirmer test, fluorescein staining, tear film break-up time and lacrimal river height measurement.Results:NF-kB and TGF-beta1 presents the trend of increased and then decreased, compared with the preoperative, postoperative 15 days in patients with NF-kB and TGF-beta1 was significantly increased, (P<0.05); Compared with operation after 15 days, the expression of NF-kB TGF-beta1 staining degree decreased, (P<0.05); Compared with the preoperative, postoperative 15 day tear break-up time was significantly shorter, fluorescein staining was significantly enhanced, (P<0.05); Compared with the preoperative, postoperative 30 days four checking results of no significant difference, P>0.05.Conclusion:Ultrasonic emulsification surgery can improve the patients NF-kB and TGF-beta1 expression positive rate, associated with postoperative retinal inflammation, affect the stability of the filmACTS.

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