| 郭倩倩 董芊芊 何悦 肖会敏 刘菲 王四旺.椒莪合剂的质量标准研究[J].,2015,15(15):2944-2949 |
| 椒莪合剂的质量标准研究 |
| Studies on the Quality Standard of Jiao'e Mixture |
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| DOI： |
| 中文关键词: 椒莪合剂 质量标准 薄层色谱法 高效液相色谱法 |
| 英文关键词: Jiaoe Mixture Quality standard TLC HPLC |
| 基金项目:陕西省科学技术研究发展计划(2008k10-02) |
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| 中文摘要: |
| 目的：检测椒莪合剂中主要化学成分含量并制订其质量标准。方法：①采用TLC 法对制剂中莪术醇、牻牛儿酮、莪术二酮及
琢-亚麻酸进行鉴别；②采用HPLC法，以Intersustain C18 色谱柱（4.6 mm× 250 mm，5.0 um），检测波长为216 nm，以乙腈- 水作
为流动相运用梯度洗脱法分别测定制剂中牻牛儿酮、呋喃二烯的含量；以乙腈-1 %醋酸溶液（90：10）为流动相，Kromasil C18 色
谱柱（4.6 mm× 200 mm，5.0 um），检测波长为205 nm，测定制剂中alpha-亚麻酸的含量。结果：①制剂中牻牛儿酮、莪术二酮及alpha- 亚
麻酸的薄层鉴别具有较好的专属性；②牻牛儿酮、呋喃二烯及alpha- 亚麻酸的线性范围与相关系数（r）分别为0.0060~0.1200 mg·
mL-1、0.9995，0.0120~0.2400 mg·g-1、0.9995，6.90~115.00 mg·g-1、0.9998；③牻牛儿酮、呋喃二烯及alpha- 亚麻酸平均加样回收率与
RSD 分别为98.65 %、0.65 %，99.02 %、0.55 %，98.49 %、0.67 %；④3批制剂中牻牛儿酮、呋喃二烯及alpha- 亚麻酸的平均含量（mg·
g-1）分别为18.42、53.87 和277.10 mg·g-1，RSD（%）分别为1.18、0.68 和1.37 %。结论：所建立的制剂中牻牛儿酮、呋喃二烯及alpha-亚
麻酸检测方法科学、合理，可用于其质量控制。 |
| 英文摘要: |
| Objective:To determine the content of main chemical components in Jiao'e mixture and establish its quality standard.Methods:① Curcumenol, Germacrone, Curdione and alpha-linolenic acid in the preparation were identified by TLC, respectively. Germacrone
and Furanodiene in the preparation were analyzed on Intersustain C18 (4.6 mm× 250 mm, 5.0 um) with acetonitrile-water as mobile
phases in gradient elution manner, and the detection wavelength was set up at 216 nm. ②The quantative analysis of alpha-linolenic acid
was carried out on a column of Kromasil C18（4.6 mm× 200 mm, 5.0 um）, using a mobile phase of acetonitrile-water (containing 1 %
acetic acid)(90:10) at the wavelength of 205 nm.Results:① The TLC of Germacrone, Curdione and 琢-linolenic acid in the preparation
showed good specificity. ② The linear range and correlation coefficient of Germacrone, Furanodiene and alpha-linolenic acid were
0.0060~0.1200 mg·mL-1, 0.9995, 0.0120~0.2400 mg·mL-1, 0.9995 and 6.90~115.00 mg·mL-1, 0.9998, respectively. ③ The average recoveries
and RSD of Germacrone, Curdione and alpha-linolenic acid were 98.65 %, 0.65 %, 99.02 %, 0.55 %and 98.49 %, 0.67 %, respectively.
④The average contents（mg·mL-1）of Germacrone, Curdione and alpha-linolenic acid were 18.42，53.87 and 277.10 mg·mL-1, coupled
with the average RSD（%）of 1.18, 0.68 and 1.37 %in the 3 batches of preparations.Conclusion:The determination method of Germacrone,
Furanodiene and alpha-linolenic acid in the preparation is so scientific and reasonable that can be used for its quality control. |
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