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目的：在妊娠过程中，胎盘可能暴露于多种病原微生物，威胁胎儿正常生长发育。为探讨人胎盘绒毛组织是否表达AIM2 炎性体成员基因以及人胎盘组织的AIM2 炎性体的活化形式。方法：以THP-1 细胞来源的RNA 和蛋白作为阳性对照，分别应用 RT-PCR和Western blot方法检测人早孕期胎盘绒毛组织中AIM2 炎性体两个相关基因AIM2 和ASC 的表达。分离和体外培养人胎盘绒毛膜组织，并用不同浓度的poly(dA:dT)进行转染，处理24小时后，分别收集组织培养上清和蛋白裂解液，Western blot 检测蛋白裂解液中caspase-1 的活化，ELISA 检测培养上清中IL-1beta的分泌。结果：RT-PCR和Western blot 结果均显示人早孕期胎盘绒毛组织组成性表达AIM2 炎性体相关基因AIM2 和ASC。同时，体外培养的人胎盘绒毛组织在转染5 ug/mL poly(dA:dT) 后，caspase-1 剪切片段p10 显著增多，培养上清中IL-1beta分泌也显著增多（P<0.01）。结论：人胎盘绒毛组织存在功能性的AIM2炎性体，能够被胞内双链DNA 活化。

英文摘要:

Objective:During pregnancy, the placenta may become exposed to microorganisms, which may pose a potential threat to the embryo/fetus well-being. This study was preformed to investigate expression and function of the AIM2 inflammasome in human placental villi.Methods:The expression of AIM2 and ASC, two components of the AIM2 inflammasome, were determined in human placental villi by RT-PCR and Western blot. RNA and protein from THP-1 cells were used as positive control. Human placental villi were isolated and cultured in vitro, then transfected with different doses of poly (dA:dT) for 24 hours. The supernatant were collected for analysis of IL-1beta secretion and tissue lysates were harvested for detection of caspase-1 activation by Western blot.Results:RT-PCR and Western blot analysis showed that ASC and AIM2 were constitutively expressed in human placental villi. Furthermore, treatment with 5 ug/mL poly (dA:dT) markedly induced IL-1beta release and increased caspase-1 activity.Conclusion:The AIM2 inflammasome is expressed and may plays an important role in human placental villi, which is activated by cytosolic double-stranded DNA stimulation.

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