| 叶永山 汤明杰 曹春琪 苏浬 张旗 李健 李强.生地黄对内毒素诱导的急性肺损伤的保护作用[J].,2015,15(14):2610-2613 |
| 生地黄对内毒素诱导的急性肺损伤的保护作用 |
| Rehmannia Glutinosa Libosch. Protects against Lipopolysaccharide-inducedAcute Lung Injury |
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| DOI： |
| 中文关键词: 生地黄 急性肺损伤 急性呼吸窘迫综合征 肿瘤坏死因子-alpha 白细胞介素-6 |
| 英文关键词: Rehmannia glutinosa Libosch ALI ARDS TNF-alpha IL-6 |
| 基金项目:国家自然科学基金项目（81173519）；北京中医药大学创新团队项目（2011-CXTD-15） |
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| 中文摘要: |
| 目的：以中医辨证论治为基础，研究生地黄对急性肺损伤/急性呼吸窘迫综合征（acute lung injury/acute respiratory distress
syndrome，ALI/ARDS）的干预作用。方法：将大鼠分为对照组、模型组、生地黄预防组、生地黄治疗组，通过气管注射脂多糖（LPS）
制备大鼠ALI/ARDS模型，酶联免疫法（ELISA）测定支气管肺泡灌洗液（bronchoalveolar lavage fluid，BALF）中肿瘤坏死因子-alpha
（tumor necrosis factor-alpha，TNF-alpha）和白细胞介素-6（interleukin-6，IL-6）含量的变化，考马斯亮蓝法测定BALF中总蛋白含量，BALF
细胞悬浮液离心涂片Giemsa 染色后光镜下细胞分类计数，H.E.染色肺部组织并通过光镜观察其形态学变化，分析生地黄水提物
对ALI的保护作用。结果：生地黄干预组BALF中TNF-alpha、IL-6 含量明显降低（P<0.0），蛋白含量明显降低（P<0.0），中性粒细胞数
（Polymorphonuclear cells，PMN）和炎症细胞总数明显降低（P<0.0），肺部病理损伤减轻。结论：生地黄对ALI可能具有保护作用，
对ALI/ARDS 可能具有潜在的治疗价值。 |
| 英文摘要: |
| Objective:The purpose of the study reveal the intervention effect of Rehmannia glutinosa Libosch., which is a chief
traditional Chinese medicine with efficacy of removing heat to cool blood based on the syndrome differentiation and treatment in Chinese
medicine, on ALI/ARDS.Methods:Rats were divided into four groups, respectively control group, model group, radix rehmanniae
prevention group and treatment group. ALI/ARDS model in rats were developed through the trachea injection of lipopolysaccharide
(LPS). cytokine levels in BALF including TNF-alpha, IL-6 were assayed using ELISA kits. The total protein concentrations in BALF were
measured using CBB ( Coomassie brilliant blue ) assay. The number of total cells and neutrophils in BALF were also measured，and
Hematoxylin and eosin (H&E) staining was conducted in assessment of histopathological changes.Results:The number of total cells and
neutrophils and the concentration of protein exudation in BALF remarkably decreased in in P and T group. Cytokine levels mentioned
above were also significantly inhibited at 12h after LPS administration in P and T group. Furthermore, leukocyte infiltration and protein
exudation in the alveolar space were less severe in the P and T group than in theMgroup.Conclusion:The findings of this study suggest
that the administration of Rehmannia glutinosa Libosch. improves ALI，possibly alleviating ALI through immunoregulation. |
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