| 曹振昊 田云虎 马璐璐 赵厚岭 毕建超.髓核突出模型大鼠背根神经节中Wnt5a的表达[J].,2015,15(13):2443-2446 |
| 髓核突出模型大鼠背根神经节中Wnt5a的表达 |
| Expression of Wnt5a in the DRG of Rats in the Model of Protrusion ofNucleus Pulposus |
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| DOI： |
| 中文关键词: 背根神经节 Wnt5a 髓核突出 TNF-alpha 依那西普 |
| 英文关键词: DRG Wnt5a Protrusion of nucleus pulposus TNF-alpha Etanercept |
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| 中文摘要: |
| 目的：观察Wnt5a 在大鼠髓核突出模型的表达变化，探索Wnt5a 在神经根病中的作用。方法：将80只成年雄性大鼠随机分
为假手术组（Sham 组，n=20）、髓核突出组（NP 组，n=20）、髓核突出+ 生理盐水组（NP+Saline 组，n=20）、髓核突出+ 益赛普组
（NP+ Etanercept 组，n=20）。从大鼠尾椎椎间盘中取髓核，将髓核种植于L5 背根神经节旁，制作髓核突出大鼠模型。采用免疫组织
化学法分别检测各组大鼠背根神经节中的Wnt5a 蛋白的表达，利用RT-PCR 的方法分别检测各组大鼠背根神经节中Wnt5a mRNA
的表达。结果：免疫组化结果显示，各组大鼠背根神经节的大中小神经元均有Wnt5a 的表达，NP 组较Sham 组在3 天、7天时
Wnt5a 表达量均增加（P<0.01），3 天和7 天时NP+Etanercept 组大鼠背根神经节中Wnt5a 的表达量较NP+Saline 组均减少（P<0.
01）。RT-PCR 的结果与免疫组化的结果相符，表现为NP 组较Sham组在3 天、7 天时Wnt5a mRNA 的相对表达量增加（P<0.01），
NP+Etanercept组大鼠背根神经节中Wnt5a的相对表达量较NP+Saline 组均减少（P<0.01）。结论：髓核突出并压迫背根神节时，可
引起背根神经节Wnt5a 的表达增加，且阻断肿瘤坏死因子琢（TNF-alpha）时，背根神经节的Wnt5a表达减少。 |
| 英文摘要: |
| Objective:To explore the expression of Wnt5a in the model of protrusion of nucleus pulposus (NP), and investigate its
role in the radiculopathy.Methods:80 adults male rats were allocated into 4 groups which contained shame group (n=20), NP group
(n=20), NP+saline group (n=20) and Etanercept group (n=20). The NP was extracted fromintervertebral disc of coccygeal vertebra of the
rats, then was implanted on the left side of the dorsal root ganglia of the fifth lumbar vertebra. After surgery, a NP model had been finished.
The expression of Wnt5a protein in the DRG was examined by immunohistochemistry (IHC). The expression ofWnt5a mRNA in
the DRG was detected by reverse transcription polymerase chain reaction (RT-PCR).Results:Wnt5a was expressed in the DRG in every
group by IHC. Compared with the shame group, it was increased markedly in the NP groupon both the 3rd day and the 7th day (P<0.01).
The expression of Wnt5a was decreased in the DRG in Etanercept group contrasted with NP+saline group on both the 3rd day and the 7th
day (P<0.01). The results of RT-PCR were coincident with that of IHC. Compared with the sham group, Wnt5a mRNA was increased
markedly in the NP group on both the 3rd day and the 7th day (P<0.01). The expression ofWnt5a mRNA was decreased in the DRG in
Etanercept group contrasted with NP+saline group on both the 3rd day and the 7th day (P<0.01).Conclusion:The expression ofWnt5a
in the DRG was increased after the compression of nerve root by the extrusive NP, while the expression of Wnt5a in the DRG was decreased
after the administration of the inhibitor of TNF-alpha. |
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