| 季蒙蒙 杨金伟 杜斌 黄玲美 郭建辉 李力燕.天麻素对胃癌细胞活力和凋亡的影响[J].,2015,15(13):2421-2424 |
| 天麻素对胃癌细胞活力和凋亡的影响 |
| Effect of Gastrodin on Activity and Apoptosis of Gastric Cancer Cells |
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| DOI： |
| 中文关键词: 天麻素 SGC-7901 凋亡 |
| 英文关键词: Gastrodin SGC-7901 Apoptosis |
| 基金项目:国家自然科学基金项目（81260075；31260253）；云南省科技厅-昆明医科大学应用基础研究联合专项（2012FB002；
2012FB092；2013FB114；2013FZ295）；云南省肿瘤转化医学工程技术研究中心（2011DH011）； |
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| 中文摘要: |
| 目的：探讨天麻素在体外对人胃癌细胞SGC-7901 细胞活力和凋亡的影响。方法：培养人胃癌细胞株SGC-7901，随机分为正
常组、对照组和实验组，再按不同时间点分为6、12、24、48、72 h 5个亚组，每个亚组8 孔细胞。实验组按2 mg/mL加入天麻素，对
照组加入等量生理盐水，正常组不作其它处理。运用MTT 法检测不同时间点细胞活力，运用TUNEL法检测细胞凋亡情况。结果：
天麻素对人胃癌细胞SGC-7901 具有增殖抑制和促进凋亡作用，并具有时间依赖关系。正常组、对照组之间细胞活力及凋亡比较
均无统计学意义（P>0.05）。在6 h、12 h、24 h、48 h、72 h时间点，实验组的细胞活性分别为0.41± 0.05、0.42± 0.07、0.43± 0.06、
0.47± 0.07、0.52± 0.08，对照组分别为0.44± 0.07、0.49± 0.12、0.50± 0.09、0.69± 0.11、0.72± 0.12，实验组细胞活力较对照组降低
（P<0.05），实验组的凋亡指数分别为7.12± 0.65、10.48± 1.26、15.37± 1.69、17.52± 2.33、33.62± 3.65，对照组的凋亡指数分别为
1.08± 0.05、3.41± 0.56、6.09± 0.91、9.52± 1.14、11.57± 1.18，实验组较对照组凋亡显著增加（P<0.05）。结论：天麻素可降低人胃癌
SGC-7901 细胞的活力并增加细胞的凋亡，可能具有抗肿瘤的作用。 |
| 英文摘要: |
| Objective:To explore the effect of Gastrodin on cell viability and apoptosis of SGC-7901 in vitro.Methods:SGC-7901
cells were cultured and then were randomly divided into normal, control and experimental groups. According to different effecting time,
they were further divided them into 6, 12, 24, 48, 72 h, 5 subgroups. Each subgroup was given 8 well cells. The experimental group received
2 mg/mL Gastrodin, control group was given saline, normal group had no other treatment. The cell viability was detected in different
time points by MTT, TUNEL method was used for apoptosis detection.Results:Gastrodin inhibited the cell proliferation and promoted
cell apoptosis, and these effects had time dependency. Cell viability and apoptosis between normal and control group had no statistical
significance (P>0.05). At 6 h, 12 h, 24 h, 48 h, 72 h time points, the cell viability of experimental group was respectively 0.41± 0.05,
0.42± 0.07, 0.43± 0.06, 0.47± 0.07, 0.52± 0.08, while that was respectively 0.44± 0.07, 0.49± 0.12, 0.50± 0.09, 0.69± 0.11, 0.72±
0.12 in control group. The cell viability of experimental group was lower than that of control group（P<0.05）. The apoptotic index of experimental
group was respectively 7.12± 0.65, 10.48± 1.26, 15.37± 1.69, 17.52± 2.33, 33.62± 3.65, while that was respectively 1.08±
0.05, 3.41± 0.56, 6.09± 0.91, 9.52± 1.14, 11.57± 1.18 in control group. Compared with control group, the apoptotic index of experimental
group was profoundly higher (P<0.05).Conclusion:Gastrodin can decrease the gastric cancer SGC-7901 cell viability and increase
cell apoptosis, which may have anti-tumor effect. |
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