| Objective:To discuss the curative effect of the co-integrated vector of AAV-PR39-ADM which secret angiogenesis
peptide (PR39) and Adrenomedullin(ADM) on a rat ischemia-reperfusion injury model.Methods:36 healthy adult male SD rats, weight
280g± 20g were randomly divided into sham-operation(SO), the treatment group (TR) and control group (I/R) each12. Ischemia-reperfusion
injury model was established in both TR group and I/R group and co-integrated vector of AAV-PR39-ADMwas injected into the injured
myocardiumof the treatment group rats. Echocardiographic measurement and record left ventricular systolic pressure (LVSP), maximal
rate of rise of ventricular pressure (+dp/dt max), maximal rate of decline of ventricular pressure (-dp/dt max) and ejection fraction
(EF %) were the treatment group rat of co-integrated vector of AAV-PR39-ADM transshipment myocardium after 7 days. The sham
group was operated without coronary artery ligation. Hearts were speedy extracted after the execution of the rats, MASSON staining was
carried out in the experiment.Results:The ejection fraction, left ventricular systolic pressure, maximum rate of left ventricular pressure
rise or fall of the treatment group were significantly higher than those in control group [EF%(50.4± 6.3), (29.8± 10.5), P<0.05; LVSP:
(116± 4.2), (101± 3.7), P<0.05; +dp/dt max: (2859± 365), (2137± 191), P<0.05; -dp/dtmax: (2186± 107), (1886± 124), P<0.05; IS%:
(29.3± 4.6), (24.6± 2.2), P<0.05.].Conclusion:Co-integrated vector of AAV-PR39-ADMsignificantly attenuated the declines of LVSP,
increased the myocardial contraction ability, improved the ejection fraction and obviously reduced infarct size. |
