| Objective:To explore the effect of PEI-CS/siRNA nanoparticles on gene expression of multidrug-resistant
hepatocellular carcinoma cell line BEL7402/5-FU in MRE11.Methods:BEL7402/5-FU was transfected with PEI-CS/siRNA
nanoparticles, which were prepared with PEI-CS (100 ug/mL) and different concentration of MRE11 siRNA-FAM by condensation
method. Then, the rate of transfection was observed by fluorescence microscopy, the silencing efficiency was assessed by Real-time PCR.Results:As shown by fluorescence microscopy, when the cell was transfected after 48 hours using nanoparticles which were prepared
with PEI-CS at different concentration of siRNA (3.125, 6.25, 12.5, 25, 50 ug/mL), the transfection efficiency showed 62.31%, 76.09%,
79.99%, 86.49%, and 96.59%, respectively. When the cell was transfected with nanoparticles at the concentration of 12.5 ug/mL for
different times (48, 72, 96 h), the transfection efficiency was 78.22%, 55.76% and 42.85%, respectively. However, under the test
conditions above, the transfection efficiency were respectively turned into 83.67%, 74.23% and 67.45%, when the concentration of
nanoparticles was 25 ug/mL. Real-time PCR results showed that the efficiency of RNA interference for MRE11 was 35.4% by
PEI-CS/siRNA nanoparticles at the concentration of 25 ug/mL.Conclusion:PEI-CS/siRNA nanoparticles can not only effectively
transfectmultidrug-resistant hepatocellular carcinoma cell line BEL7402/5-FU, but also down-regulate expression levels of geneMRE11. |
