| 武子朝 韩瑞旸 朱建彪 魏煜军 魏龙晓.丹酚酸B 通过抑制线粒体功能增加胶质瘤细胞的放疗敏感性[J].,2015,15(9):1636-1639 |
| 丹酚酸B 通过抑制线粒体功能增加胶质瘤细胞的放疗敏感性 |
| Salvianolic Acid B Renders Glioma Cells More Sensitive to Radiation viaInhibiting Mitochondrial Function |
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| DOI： |
| 中文关键词: 丹酚酸B 放疗 线粒体 胶质瘤 |
| 英文关键词: SalB Radiation Mitochondria Glioma |
| 基金项目:国家自然科学基金面上项目（81371447） |
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| 中文摘要: |
| 目的：研究药用植物丹参的根茎提取成分丹酚酸B对人胶质瘤U251 细胞的放疗增敏作用，并探讨其可能的分子机制。方
法：使用1 μM浓度的丹酚酸B 处理胶质瘤U251 细胞，并用等量PBS 建立对照组，使用射线照射建立放射治疗模型。MTT 法检
测细胞活力；流式细胞术检测细胞凋亡；荧光染色检测活性氧ROS含量及线粒体肿胀程度。结果：丹酚酸B 能够显著降低射线照
射后U251 细胞活力，并增加其凋亡（P<0.05）。丹酚酸B 能够显著增加射线照射后U251 细胞活性氧ROS的产生，并增加线粒体
的肿胀程度（P<0.05）。结论：丹酚酸B 能够通过诱导内源性凋亡增加胶质瘤细胞的放疗敏感性，这种作用可能是通过抑制线粒体
功能而实现的。 |
| 英文摘要: |
| Objective:To investigate the effects of salvianolic acid B (SalB) on the radiosensitization of human glioma U251 cells
and the possible underlying mechanisms.Methods:U251 cells were treated with 1 μM SalB or phosphate buffered saline (PBS), and
treated with radiation to establish the in vitro model. MTT assay was used to measure the cell viability; flow cytometry was used to detect
the apoptotic cell death; fluorescence staining was used to measure the generation of reactive oxygen species(ROS) and the mitochondrial
swelling in U251 cells.Results:SalB significantly decreased cell viability and increased apoptotic cell death after radiation in U251 cells
(P<0.05). SalB significantly increased intracellular ROS generation and mitochondrial swelling after radiation in U251 cells (P<0.05).Conclusion:Salvianolic acid B renders glioma U251 cells more sensitive to radiation through inducing intrinsic apoptotic cell death, and
these effects was partly dependent on the inhibition of mitochondrial function. |
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