孙全波 栾晓 郭菲菲 孙向荣 祝海 逄明杰 王萍 谷方 徐珞.Obestatin 对血浆ghrelin和nesfatin-1水平及胃排空的影响[J].,2015,15(9):1628-1631 |
Obestatin 对血浆ghrelin和nesfatin-1水平及胃排空的影响 |
The Effects of Obestatin on Plasmaacyl Gheelin and Nesfatin-1 Levels,and on Gastric Emptying in Rats |
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DOI: |
中文关键词: 胃排空 Ghrelin Nesfatin-1 Obestatin |
英文关键词: Gastric emptying Ghrelin Nesfatin-1 Obestatin |
基金项目:国家自然科学基金项目(31071014;81100260;81300281;81470815;81270460);青岛市科技局项目(13-1-4-170-jch;14-2-3-3-nsh) |
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中文摘要: |
目的:探讨侧脑室注射obestatin 对大鼠血浆酰基化ghrelin、去酰基化ghrelin、nesfatin-1 水平的影响以及对胃排空的调控。方
法:侧脑室注射obestatin,采用酶免疫测定(EIA)法检测血浆酰基化ghrelin、去酰基化ghrelin、nesfatin-1 水平以及胃排空率的变
化。结果:侧脑室分别注射0.1、0.3 或1.0 nmol obestatin,大鼠血浆酰基化ghrelin、去酰基化ghrelin 以及nesfatin-1 水平无显著改
变(P>0.05),且酰基化ghrelin 与去酰基化ghrelin 比率无显著改变(P>0.05);侧脑室注射obestatin,大鼠摄食量无显著改变,但胃
排空率明显增加(P<0.05);胃排空率明显延迟(P<0.05)。与侧脑室注射1.0 nmol Obestatin 组相比,注射1.0 nmol Obestatin+CRF,
大鼠摄食量无显著改变,胃排空率明显延迟(P<0.05)。各组摄食量及进入十二指肠内食物量无明显差异(P>0.05)。结论:中枢
obestatin 促进大鼠的胃排空,可能与h/rCRF通路有关。 |
英文摘要: |
Objective:To discuss the impact of intracerebroventricular obestatin on plasma acyl ghrelin, des-acyl ghrelin and
nesfatin-1 levels, and the regulation of gastric emptying in rats.Methods:Rats were administrated obestatin through
intracerebroventricular (ICV) injection; Enzyme immunoassay (EIA) method was used to detect the plasma levels of acyl ghrelin,
des-acyl ghrelin, nesfatin-1 and changes in the rate of gastric emptying.Results:After lateral ventricle injection respectively 0.1, 0.3 or
1.0 nmol obestatin, the plasma levels of acyl ghrelin, des-acyl ghrelin, nesfatin-1 had no significant change (P>0.05), and the ratio of acyl
ghrelin and des-acyl ghrelin had no significant difference (P>0.05) too. Food intake of rats had no obvious difference after lateral
ventricle injection of obestatin, but the gastric emptying rate increased significantl (P<0.05). Gastric emptying rate delayed significantly
(P<0.05). Compared with lateral ventricle injection 1.0 nmol Obestatin group, injection 1.0 nmol Obestatin + CRF, no significant changes
in food intake in rats significantly delayed gastric emptying rate (P<0.05). While the amount of food intake and the food into the
duodenum had no obvious difference (P>0.05).Conclusion:Central obestatin promotes gastric emptying of rats, which may be
associated with h/rCRF pathways. |
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