| 王沛坚 黄鸣清 蒲春华 王芳 王秋林 周鹏.姜黄素激活 AMPK 抑制高糖介导的氧化应激水平升高致血管内皮功能
障碍[J].,2015,15(7):1237-1240 |
| 姜黄素激活 AMPK 抑制高糖介导的氧化应激水平升高致血管内皮功能
障碍 |
| Curcumin Suppressed Oxidative Stress and Improved EndotheliumDysfunction through AMPK Activation |
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| DOI： |
| 中文关键词: 姜黄素 氧化应激 高糖 AMPK 血管内 皮 |
| 英文关键词: Curcumin Oxidative stress High glucose AMP-Activated Protein Kinase Endothelial |
| 基金项目:国家自然科学基金项目（ 81 373940）； 四川省教育厅科研项目（ 14ZB0234）； 成都医学院科研项目（ CYZ1 3-001） |
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| 中文摘要: |
| 目的： 探索姜黄素对高糖介导的血管氧化应激水平的升高和血管功能的保护作用 未知及相关的机制 。 方法： 以高糖 DMEM
培养基复制人脐静脉内 皮细胞（ HUVECs）的高糖损害模型。 姜黄素干预 24h 后以 DHE 荧光探针分析姜黄素对高糖介导的活性氧
产生的影响； 分离 健康 C57BL/6J 小鼠胸主动脉， 以高糖 DMEM 培养基进行体外培养制 作高糖介导的血管损害模型，分析姜黄素
对高糖介导的血管功能的影响。利用 western blotting 方法检测血管内 皮细胞中 p-eNOS 及 p-AMPK 的表达水平。 结果： 姜黄素可
显著减少高糖介导的血管内 皮细胞活性氧水平， 该作用 在给予 AMPK 抑制剂 后显著降低； 姜黄素可显著促进高糖环境血管内 皮
细胞 eNOS 的磷酸化并可显著改善高糖环境下小鼠胸主动脉的血管内 皮依赖性舒张功能。 Western blotting 结果表明， 姜黄素可显
著增加 p-AMPK 的表达。 结论： 姜黄素显著减轻高糖血管内 皮细胞的氧化应激水平， 改善血管内 皮依赖性舒张功能， 而该作用 可
能与 其通过激活 AMPK/eNOS 通路有关。 |
| 英文摘要: |
| Objective:To investigate the effect and mechanism of curcumin on elevating of oxidative stress and endothelial dysfunction induced by high glucose.Methods:The impaired Human Umbilical Vein Endothelial Cells （ HUVECs）model was induced by
cultured with high glucose DMEM (33mM D-glucose). The ROS level was detected by DHE fluorescent probe 24h after treated with or
without curcumin. Aorta from C57BL/6J mice were isolated and cultured with high glucose DMEM, low glucose DMEM, high glucose
DMEM plus curcumin or high glucose plus curcumin and compound C respectively for 24hrs. Wire myograph was used to analyze the
vascular function. And phosphorylation of eNOS and AMPK was detected by western blotting.Results:Curcumin ameliorated the ROS
level induced by high glucose, and Com C reversed this situation. Cultured with curcumin increased p-eNOS level and improved the endothelium dependent relaxation significantly but decreased the endothelium independent relaxation under high glucose condition, these
results also blocked by cultured with Com C. Curcumin increased the p-AMPK level of high glucose treated ECs.Conclusion:Curcumin
attenuated ROS level and improved endothelium dependent relaxation in high glucose treated ECs and aortic, these effects may be due to
the activation of AMPK/eNOS pathway. |
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