| 陈文娟 刘亮 胡道德 刘皋林.5- 氨基水杨酸纳米粒对 TNBS 诱导小鼠结肠炎的治疗作用[J].,2015,15(7):1206-1208 |
| 5- 氨基水杨酸纳米粒对 TNBS 诱导小鼠结肠炎的治疗作用 |
| Effects of 5-ASA Nanoparticles on TNBS-Induced Colitis in Rats |
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| DOI： |
| 中文关键词: 5-ASA 纳米粒 结肠炎 髓过氧化物酶 |
| 英文关键词: 5-ASA Nanoparticles Colitis MPO |
| 基金项目:上海市科委纳米技术专项基金（ 1052nm04000）；上海交通大学医工交叉基金（ 090YG016） |
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| 中文摘要: |
| 目的： 评价超临界分散法制备的 5- 氨基水杨酸（ 5-ASA）结肠定位纳米粒对小鼠结肠炎的治疗作用 。 方法： 将 24 只 6 周 龄
BABL/c 小鼠随机分为空白对照组， 结肠炎模型组， 5-ASA 治疗组， 5-ASA 纳米粒治疗组。 三硝基苯磺酸（ TNBS）造模后的第 2-7 d
分别给予 0.9%氯化钠， 5-ASA 和 5-ASA 纳米粒灌胃 ， 于第 8 d 处死所有小鼠，评估小鼠的疾病活动指数(DAI) ，组织病理形态以
及结肠组织学损伤情况；检测结肠组织髓过氧化物酶(MPO)活性。 结果： 5-ASA 和 5-ASA 纳米粒均能明显减轻结肠重量， 缩小溃
疡面积， 降低 MPO 活性。 超临界分散法制备的 5-ASA 纳米粒在 DAI， 减轻局部的炎症程度(MPO 水平)方面， 其疗效与 5-ASA 组
（ 50 mg/Kg）相当。 结论： 超临界分散法制备的 5-ASA 纳米粒对 TNBS 诱导小鼠的结肠炎有明显的治疗作用 。 |
| 英文摘要: |
| Objective:To evaluate the effects of 5-ASA-nanoparticles (NP) on TNBS-induced colitis in rats.Methods:Twenty-four BALB/c rats were randomized into normal control group, colitis group, 5-ASA group, and 5-ASA-NP group, with six rats in each
group. 5-ASA, 5-ASA-NP and normal saline solution were oral administrated respectively on day 2-7 after the establishment of colitis.
On day 8, all animals were sacrificed. The change of body weight, weight to length ratio of colon, and macroscopic and pathologic score
of colon were assessed. MPO activity of colonic tissue was measured.Results:Compared with normal group, the DAI, macroscopic and
pathologic scores, MPO activity in colonic tissue in TNBS-control group increased significantly. In rats treated with 5-ASA or
5-ASA-NP, all the above-mentioned indices decreased significantly, and efficacy of 5-ASA-NP was almost the same as 5-ASA (50
mg/Kg).Conclusion:5-ASA-NP prepared by supercritical dispersion has significant effect on TNBS-induced colitis in rats with lower
side effects, which suggested that it might be a potential therapeutic strategy for IBD. |
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