| 刘 艳艳 张 凯 关家伟 曹雪姣 吴大畅.人参皂苷对 BALB/c 小鼠肠道菌群的影响[J].,2015,15(6):1041-1045 |
| 人参皂苷对 BALB/c 小鼠肠道菌群的影响 |
| The Effects of Ginsenoside on the Intestinal Microbiota of Mice |
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| DOI： |
| 中文关键词: 人参皂苷 肠道菌群 PCR-DGGE |
| 英文关键词: Ginsenoside Intestinal microbiota PCR-DGGE |
| 基金项目: |
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| 中文摘要: |
| 目的： 随着人参药用和保健价值的不断发掘， 人参皂苷引起人们越来越多 的关注，但有关人参皂苷与 肠道菌群之间相互作用
的研究仍为空白领域， 本实验旨在探明人参皂苷对小鼠肠道菌群的影响，以期为人参皂苷的推广应用 提供理论基础和实验依据。
方法： 有机溶剂 法提取人参皂苷， 将正常 BALB/c 小鼠按 2 mg/0.1 kg 人参皂苷进行连续灌胃 饲养， 分别 在灌胃 第 10 d 和第 1 3 d
无菌收集小鼠粪便， 提取肠道细菌基因组总 DNA， 应用 PCR-DGGE 技术获得肠道菌群分子指纹图 谱， 进行菌群结构相似性、多 样
性分析， 并将感兴趣的优势条带进行切胶、测序分析，对获得的序列 在 GeneBank 数据库比对。 结果： 灌胃人参皂苷后小鼠肠道菌
群结构发生改变， 荧光假单胞菌和丁酸梭菌数量明 显增加。 结论： 人参皂苷使小鼠肠道的菌群结构和数量发生明 显改变， 天然的
人参皂苷口服很难被直接吸收利 用 ，因 此推测人参皂苷可能以肠道菌群作为 发挥生物学作用的靶点， 进而行使提高健康水平等
保健功能。 |
| 英文摘要: |
| Objective:People pay more attention to ginsenoside with the investigation of medicinal and health value of ginseng,
but the interaction between ginsenoside and intestinal microbiota still keep as a blank. This experiment aim to explore the effects of
ginsenoside on the intestinal microbiota of mice, so as to provide theoretical and experimental basis for the popularization and application
of ginsenosides.Methods:The ginsenoside was extracted through the organic solvent extraction process. Then the ginsenoside was given
to the normal BALB/c mice at 2 mg/0.1 kg for ten days, fresh feces samples were collected at 10 d, 13 d under aseptic condition,
respectively. After extraction of total bacterial genomic DNA from feces, index analyses of diversity， similarity were carried out through
DGGE profiling obtained by PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) technique. And the interest
bands in DGGE profile were cut off and sequencing analysis, to obtain the sequence alignment in GeneBank database.Results:There
were great changes in the intestinal microbial community structure after the intervene of ginsenoside. Further DNA sequence analysis
indicated that there were a remarkable increase in the number of and .Conclusion:Ginsenoside made the structure and quantities changed in intestinal microbiota of mice, and the natural ginsenosides are difficult directly
absorpt after oral administration, so ginsenoside may take the intestinal microbiota as the target to act its biological functions, so as to
improve the health level and some related health care functions. |
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