| Objective:Testing the changes of superoxide dismutase(SOD) and malondialdehyde (MDA) in rats with diffuse brain
injury (DBI) or secondary brain insult (SBI), to analyze the effects and significance of peroxidation in development of rats SBI.Methods:90 male SD rats were randomly divided into normal control group (group A), DBI group (group B) and SBI group (group C). After the
success of the modeling, the rats were put to death, and the frontal tissue homogenate were taken after injury 1 h, 3 h, 6 h, 12 h, 24 h, and
48 h respectively, to determine the content of SOD and MDA.Results:From the first 1 h after injury, SOD content in B and C groups
presents the downward trend after rising first, and the declining trend over time until 24 h after injury, the degree of decline in group C is
more obvious compared with that in group B (P< 0.05); 3 h after injury, SOD content in B and C groups were higher, the higher degree
and the rate of B group were higher than in group C (P<0.05). From the first 1 h after injury, MDA content in groups B and showed a
trend of increase, and in 24 hours to spike, group C increased more significantly than group B, at 6 h, 24 h, and 48 h after injury, the differences between Group B and Group C were statistically significant (P<0.05).Conclusion:After DBI or SBI in rats, peroxidation increase obviously, SOD and MDA change ap- parently, peroxidation in SBI is more serious than that in DBI and last longer, and the
changes of MDA are later than those of SOD. |
