| 刘吉耀 赵波 姚向荣 廖军.脑梗塞大鼠脑组织中Rho 激酶活性变化及其临床意义[J].,2015,15(3):445-448 |
| 脑梗塞大鼠脑组织中Rho 激酶活性变化及其临床意义 |
| The Study of Rho Kinase Activity in Brain Tissuein the Cerebral Infarction in Rats |
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| DOI： |
| 中文关键词: Rho 激酶 脑梗塞 MBS 大鼠 |
| 英文关键词: Rho kinase Cerebral infarction MBS Rat |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨脑梗塞过程中大脑组织中的Rho 激酶活性变化，揭示其潜在的临床价值。方法：大鼠左侧颈内动脉内注射月桂酸
钠，诱导同侧脑半球发生脑梗塞，建立大鼠脑梗塞模型。在注射月桂酸钠前及注射后0.5h，3h 及6h 四个时间点上各处死大鼠6
只，取双侧大脑实质组织，用于组织匀浆提取蛋白，通过ELISA 法检测大鼠大脑实质组织中Rho 激酶的活性。另取6h 组大鼠的大
脑组织用免疫荧光染色法检测Rho 激酶底物-MBS 的磷酸化情况。结果：在注射月桂酸钠之后3h 及6h，同侧大脑实质（即梗塞
侧）内的Rho 激酶活性明显高于对侧正常脑实质内的Rho 激酶活性（P<0.05），但在注射后0.5h内，双侧的Rho 激酶活性无明显
差异。结论：在大鼠脑梗塞模型中，大脑神经元内的Rho 激酶活性能明显被活化，提示Rho 激酶可能成为脑梗塞治疗的一个重要
靶标。 |
| 英文摘要: |
| Objective:To investigate the Rho-kinase activity in brain of the rat cerebral infarction model.Methods:Cerebral
infarction rat model was made by injecting the sodium laurate into the left internal carotid artery to induce the infraction in the ipsilateral
hemisphere. At 6 hours before injection, and at 0.5 h, 3 h, 6 h after injection of laurate, 6 rats were put to death respectively, and then the
Rho-kinase activity in extracts of the cerebral hemispheres was measured by an ELISA method. The phosphorylation MBS in the brain of
6 hours' rats were detected by immunofluorescent staining.Results:Rho-kinase activity in extracts of the ipsilateral hemisphere
significantly increased, compared with that in extracts of the contralateral hemisphere, at 3 h and 6 h but not 0.5 h after injection of
laurate.Conclusion:These findings indicated that Rho-kinase was activated in neuronal cells in a rat cerebral infarction model, and
suggested that Rho-kinase could be an important target in the treatment of cerebral infarction. |
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