| 王乐 赵兴春 张建 陈婷 王邦义 白雪 叶健.四川省夹江县人群18 个STR 位点的遗传多态性[J].,2015,15(1):126-130 |
| 四川省夹江县人群18 个STR 位点的遗传多态性 |
| Genetic Polymorphismof 18 Short TandemRepeat Loci in JiajiangCounty of Sichuan Province |
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| DOI： |
| 中文关键词: 法医遗传学 DNATyperTM19 STR 遗传多态性 |
| 英文关键词: Forensic genetics DNATyperTM19 STR Genetic polymorphism |
| 基金项目:中央级公益性科研院所基本科研业务费重大项目（2012JB001） |
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| 中文摘要: |
| 目的：利用DNATyperTM19 试剂盒研究18 个短串联重复序列（Short Tandem Repeat，STR）位点（D5S818、D21S11、D7S820、
CSF1PO、D2S1338、D3S1358、vWA、D8S1179、D16S539、Penta E、TPOX、TH01、D19S433、D18S51、FGA、D6S1043、D13S317、D12S391）
在四川省夹江县人群中的基因频率分布和群体遗传学参数，并计算DNATyperTM19 试剂盒的相关技术参数。方法：利用血卡直接
作为模板，采用PCR扩增和毛细管电泳检测技术对226 名个体的18 个STR基因座进行分析，并使用PowerStatsV12 软件统计分
析。结果：共检出202 种等位基因，基因频率分布在0.002～0.527 之间。18 个STR基因型分布均符合Hardy-Weinberg 平衡（P >
0.05），杂合度均不低于0.633，随机匹配概率均不低于0.018，个人识别能力均不小于0.790，多态信息含量均不小于0.56，非父排
除概率均不小于0.332，典型父权指数均不小于1.36。结论：本文研究了四川省夹江县人群18个STR位点的遗传多态性，为人类
群体遗传学及法医学后续研究提供详实可靠的基础数据。DNATyperTM19 试剂盒的累积随机匹配概率达到3.477× 10-22，累积非父
排除概率为0.999999974。 |
| 英文摘要: |
| Objective:The aim of this study was to determine the allele frequencies and population genetic parameters of 18 STR
(short tandem repeat) loci (D5S818, D21S11, D7S820, CSF1PO, D2S1338, D3S1358, vWA, D8S1179, D16S539, Penta E, TPOX,
TH01, D19S433, D18S51, FGA, D6S1043,D13S317, and D12S391) in Jiajiang county of Sichuan province and to calculate the technical
parameters of the DNATyperTM19 kit.Methods:PCR amplification using blood as template directly and capillary electrophoresis technologies
were employed to determine the genotypes of 18 STR loci for 226 individuals. PowerStatsV12 software was used for analysis
and statistics.Results:202 alleles were recognized, with frequencies ranging from 0.002 to 0.527. No departures from Hardy-Weinberg
expectations were detected for all 18 loci studied (P>0.05). The statistical analysis of 18 STR loci showed the heterozygosity ≥ 0.633, the
matching probability ≥ 0.018, the discrimination power ≥ 0.790, the polymorphic information content ≥ 0.56, the probability of paternity
exclusion ≥ 0.332, and the typical paternity index ≥ 1.36.Conclusion:This work studied the genetic polymorphism of 18 short tandem
repeat loci in Jiajiang county of Sichuan province providing pronounced basic data for subsequent human population genetic and
forensic DNA research work. The cumulative matching probability of the DNATyperTM19 kit reaches 3.477× 10-22, while its cumulative
excluding probability of paternity is 0.999999974. |
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