| 王兴凯 徐晓静 郑彦文 王春燕 张焕相.不同分化状态下间充质干细胞向血管内皮生长因子的迁移[J].,2015,15(1):33-38 |
| 不同分化状态下间充质干细胞向血管内皮生长因子的迁移 |
| The Migration of Differenting MSCs towards VEGF |
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| DOI： |
| 中文关键词: 细胞生物学 间充质干细胞（MSCs） 成神经分化 VEGF 细胞迁移 |
| 英文关键词: Cell Biology Mesenchymal StemCells (MSCs) Neuronal Differentiation VEGF Cell Migration |
| 基金项目:国家自然科学基金项目（31071220） |
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| 中文摘要: |
| 目的：探讨间充质干细胞(MSCs)对趋化因子VEGF的定向迁移能力与其分化状态之间的关系。方法：本实验运用采用Percoll
分离法在体外培养并扩增大鼠骨髓来源MSCs，应用抗氧化剂诱导方案诱导MSCs向神经样细胞分化，运用Boyden chamber 及
Dunn chamber趋化性迁移装置研究了在趋化因子VEGF诱导下不同分化状态的间充质干细胞定向迁移，比较了各分化状态下细
胞的迁移速度和迁移效率。结果：Boyden chamber 实验结果显示下室加入不同浓度VEGF后，不同状态细胞向同一浓度VEGF迁
移的数量不同，不同浓度VEGF 诱导同一状态细胞的迁移数量也不同；Dunn chamber的实验结果显示在某一分化阶段（预诱导24
小时）的MSCs具有更高的迁移效率。结论：MSCs的分化影响了其向VEGF的定向迁移，也就是说，不同分化状态的MSCs 显示
出不同的迁移行为。 |
| 英文摘要: |
| Objective:To investigate the relationship between VEGF-induced chemotactic migration of MSCs and their differentiation
states.Methods:MSCs were isolated by Percoll gradient centrifugation from rat and induced to neuron-like cells. Then, Boyden
chamber and Dunn chamber were used to study the behavior of direct migration fromthe population level and single cell level respectively.Results:Data from Boyden chamber experiment revealed that the migration number of cells differed among the differentiation states and
the concentration of VEGF also affected the number of migrating cells. Meanwhile, time-lapse video analysis revealed MSCs at certain
stage (24 h pre-induction) possess higher FMI (forward migration index).Conclusion:Differentiation of MSCs influences their chemotactic
responses to VEGF. |
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