| 刘玠1 曲海影1 张迪2 初李佳娃1 常越.HPLC 法测定抗宫炎凝胶剂中金石蚕苷含量的研究[J].,2014,14(36):7154-7159 |
| HPLC 法测定抗宫炎凝胶剂中金石蚕苷含量的研究 |
| Quantitative Study of Poliumoside by HPLC in the Kanggongyan Gels |
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| DOI： |
| 中文关键词: 抗宫炎凝胶剂 高效液相色谱法 金石蚕苷 |
| 英文关键词: KangGongyan gel HPLC Poliumoside |
| 基金项目:黑龙江省教育厅科学技术研究项目(12511515) |
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| 中文摘要: |
| 目的：采用高效液相色谱法(HPLC)测定抗宫炎凝胶剂中金石蚕苷的含量，为制定其质量标准提供基础。方法：采用十八烷基
硅烷键合胶为填充剂，乙腈-0.5%磷酸(18:82)为流动相；检测波长为332 nm；流速为1.0 ml/min，探讨HPLC 测定抗宫炎凝胶剂中
金石蚕苷含量的专属性、精密度、稳定性、重现性等。结果：金石蚕苷在0.104~0.468 mg/ml范围内呈良好的线性关系(r=0.9999)，平
均回收率为99.76%，RSD为1.56%。结论：采用HPLC 测定抗宫炎凝胶剂中金石蚕苷含量的专属性、精密度、稳定性、重现性均良
好，该方法简便可靠，可用于抗宫炎凝胶剂的质量标准的研究。 |
| 英文摘要: |
| Objective:To establish a HPLC method to determine the contents of Poliumoside in the Kanggongyan Gels and
provide foundation for the development of its quality standard.Methods:C18 was used as chromatographic column, the mobile phase
was Acetonitrile-0.5%H3PO4(18:82), the wave lenghth was 332 nm. and the flow rate was 1.0ml/min. The specificity, precision, stability,
and reproducibility of HPLC in the detection of content of Poliumoside in the Kanggongyan Gels were analyzed.Results:The
Poliumoside showed good linear relationship in the range of 0.104～0.468 mg/ml and the average recovery was 99.76% with RSD of
1.56% .Conclusion:The specificity, precision, stability, reproducibility of HPLC in the detection of content of Poliumoside in the
Kanggongyan Gels were good. HPLC was a simple and reliable method for the research of quality standard of kanggongyan gels. |
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