| 余震 余州 吴骐而 石定 岳毅刚 彭湃.不同剂量链尿佐菌素诱导 2 型糖尿病大鼠模型的研究[J].,2014,14(35):6835-6838 |
| 不同剂量链尿佐菌素诱导 2 型糖尿病大鼠模型的研究 |
| Study on Type 2 Diabetic Rat Models induced by Different Dosesof Streptozotocin |
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| DOI： |
| 中文关键词: 2 型糖尿病 动物模型 链脲佐菌素 |
| 英文关键词: Type 2 diabetes mellitus Animal model Streptozotocin |
| 基金项目:国家自然科学基金面上项目（ 81 272118）；国家自然科学基金面上项目（ 30973752） |
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| 中文摘要: |
| 目的： 探讨不同 剂量链脲佐菌素(Streptozotocin， STZ)联合高糖高脂饮食对 2 型糖尿病大鼠模型建立的影响。方法： 90 只 8 周
龄 SD 雄性大鼠随机平均分为 六组： 普通饲料喂养 + 缓冲液组、高糖高脂饲料喂养 + 缓冲液（ H.E 组）、高糖高脂饲料喂养 +35
mg/kg 链尿佐菌素组（ H.E+35 mg/kg STZ 组）、高糖高脂饲料喂养 +45 mg/kg 链尿佐菌素组（ H.E+45 mg/kg STZ 组）、高糖高脂饲料
喂养 +55 mg/kg 链尿佐菌素组（ H.E+55 mg/kg STZ 组）及高糖高脂饲料喂养 +65 mg/kg 链尿佐菌素组(H.E+65 mg/kg STZ 组)， 高
糖高脂饲料喂养 4 周 后诱导胰岛素抵抗，继之腹腔注射 STZ，建立 2 型糖尿病大鼠模型。 检测体重、胰岛素、空腹血糖、血脂、胰岛
素敏感指数（ ISI）。 结果： 与常规饮食组相比， 高糖高脂饮食各组大鼠出现空腹血浆胰岛素（ FINS）、空腹血糖（ FBG）、血清甘油三脂
（ TG）、总胆固醇（ TC）、游离脂肪酸（ FFA）显著升高（ P<0.01 ）， ISI 显著下降（ P<0.01）。 不同 剂量 STZ 注射， H.E+45 mg/kg STZ 组成
模率最高且无自愈现象。 结论： 通过 STZ 腹腔注射联合高糖高脂饮食可成功复制出实验性 2 型糖尿病动物模型， 45 mg/kg 为 STZ
理想注射剂量。 |
| 英文摘要: |
| Objective:To investigate the effects of different doses of streptozotocin and high-glucose-high-fat diet on the
establishment of type 2 diabetic rat model.Methods:Ninety 8-month-old SD male rats were randomly divided into six groups： Normal
feeding group, high-glucose-high-fat feeding group (H.E group), and high-glucose-high-fat+STZ group which were intraperitoneal injected
STZ with a dose of 35 mg/kg, 45 mg/kg, 55 mg/kg, and 65 mg/kg, respectively (H.E+35/45/55/65 mg/kg_STZ group). High-glucosehigh-fat diet feeding for four weeks to induce insulin resistance, then streptozotion was injected intraperitoneally to establish the type 2
diabetes mellitus model. The changes of body weight, blood insulin level, fasting blood glucose level, blood lipid level, and insulin
sensitivity index (ISI) were measured and statistically analyzed.Results:Compared with the control group, the fasting plasma insulin,
fasting blood glucose, serum triglyceride (TG), total cholesterol (TC) and free fatty acids (FFA) of H.E group and H.E ＋ STZ group
significantly increased (P<0.01 ) and the ISI significantly decreased (P<0.01 ). From different doses of STZ injection, the 45 mg/kg STZ
injection had the highest successful rate and effective stability to establish the models.Conclusion:High-glucose-high-fat diet combined
with STZ injection can successfully replicate the experimental animal models of type 2 diabetes, and 45 mg/kg is an ideal dose for STZ
injection. |
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