| 周奕 张剑峰 卢璨 彭凤兰 艾卫敏 雷德亮.两种阿尔茨海默病转基因小鼠模型脑新皮质区A(beta)分布的比较研究[J].,2014,14(34):6625-6628 |
| 两种阿尔茨海默病转基因小鼠模型脑新皮质区A(beta)分布的比较研究 |
| Comparison of the Distribution of A(beta)Immunoreactive in the Neocortex ofthe Two Kinds of Transgenic Mice Model of Alzheimer's Disease |
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| DOI： |
| 中文关键词: 阿尔茨海默病 转基因 新皮质区 beta淀粉样蛋白 |
| 英文关键词: AD Transgenic Neocortex A beta |
| 基金项目:长沙市2013 年度指导性科技计划(K13ZD046-33)；湖南省卫生厅科研基金课题（B2014-151）； |
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| 中文摘要: |
| 目的：比较A(beta)在两种阿尔茨海默病转基因小鼠模型脑新皮质区分布的差异。方法：采用18 月龄雄性APP／PSl 双转基因
(2× Tg-AD)小鼠与同龄同性别APP／PSl／tau 三转基因(3× Tg-AD)小鼠，取新皮质区脑组织行6E10 单克隆抗体免疫组化染色等方法显示A(beta)阳性神经元及斑块，观察其分布与形态等的差异，图像分析系统定量比较其量的变化。结果：在新皮质区2×Tg-AD组A(beta)阳性产物主要位于细胞外即细胞外A(beta)(eA(beta))，形成大量的老年斑，细胞内阳性产物少；而3× Tg-AD 组A(beta_阳性产物主要位于神经元细胞内即细胞内A(beta)(iA(beta))，但老年斑少见。结论：2× Tg-AD组与3× Tg-AD组A(beta)阳性产物在新皮质区分布的差异可能反映了两种AD小鼠模型神经病理等改变的不同。 |
| 英文摘要: |
| Objective:To compare the distribution and morphological features of A(beta)Immunoreactive products in the neocortex
of the APP／PSl (2× Tg-AD) and APP／PSl／tau (3× Tg-AD) model of Alzheimer’s disease.Methods:18-month old mice of 2×
Tg-AD and the age-matched mice of 3× Tg-AD were included in this experiment. In the neocortex, 6E10 immunohistochemical staining
was applied to show the distribution and morphological features of A(beta)immunoreactive products, and image analysis system was applied
to quantitatively analyze the difference of the products.Results:The deposition of A(beta)in the neocortex of 2× Tg-AD was mainly seen as
extracellular A beta(eA beta), and formed a lot of positive plaques. The positive products were distributed as intracellularly A beta(iA beta) for the
3× Tg-AD, but the A beta positive plaques were rarely seen.Conclusion:The differences of distribution of A beta positive products in the
neocortex between the 2× Tg-AD and 3× Tg-AD mice likely suggested the differences of neuropathological changes of the two AD
models. |
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