| 于俊民 鞠礼 王慧冬 万晓京 杨敬生 王勤 张小波.三七皂苷R1 对大鼠缺血心肌VEGF、bFGF的影响[J].,2014,14(30):5845-5848 |
| 三七皂苷R1 对大鼠缺血心肌VEGF、bFGF的影响 |
| Effects of Notoginsenoside R1 on VEGF and bFGF Expressionin Ischemic Myocardiumof Rats |
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| DOI： |
| 中文关键词: 三七皂苷R1 缺血心肌 血管新生 VEGF bFGF |
| 英文关键词: Notoginsenoside R1 Ischemic myocardium Angiogenesis VEGF bFGF |
| 基金项目:黑龙江省中医药科研资助项目（ZHY10-Z94） |
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| 中文摘要: |
| 目的：探讨三七皂苷R1 对大鼠缺血心肌VEGF、bFGF的影响。方法：选择雄性Wistar 大鼠39 只，建立心肌梗死（AMI）模
型，术后24h 存活大鼠随机分为药物组（n=13）、对照组（n=13），另设假手术组（n=8）。药物组给予三七皂苷R1 水溶液（2.5 mg·
kg-1·d-1）腹腔注射、对照组及假手术组给予等体积生理盐水腹腔注射，用药4 周。于实验终点处死大鼠，心肌组织取材，Ⅷ因子染色
计数微血管数（MVC）及微血管密度（MVD），免疫组织化学法观察缺血心肌VEGF、bFGF蛋白的表达。结果：药物组及对照组
MVC、MVD 均高于假手术组，且药物组高于对照组（P<0.05）；大鼠缺血心肌药物组及对照组VEGF、bFGF蛋白表达均高于假手
术组（P<0.05），且药物组高于对照组（P<0.05）。结论：三七皂苷R1 促进大鼠缺血心肌血管再生同时可上调缺血心肌VEGF、bFGF
蛋白水平。 |
| 英文摘要: |
| Objective:To study the effects of notoginsenoside R1 on the levels of vascular endothelial growth factor (VEGF) and
basic fibroblast growth factor (bFGF) in ischemic myocardiumof rats.Methods:The myocardial infarction (MI) model was established in
thirty-nine male Wistar rats. 24h later, the surviving rats were randomly divided into drug group (n=13), control group (n=13), and
sham-operated group (n=8). Rats in the drug group were injected with notoginsenoside R1 (2.5 mg·kg-1·d-1) intraperitoneally, while the
control group and the sham-operated group received same volume of saline intraperitoneally. 4 weeks after treatment, the rats were
euthanized for myocardial tissue samples. The miniature blood vessel (MVC) and microvascular density (MVD) were quantified
according to F Ⅷ staining, and the expression of VEGF and bFGF were detected by immunohistochemistry.Results:MVC and MVD
were increased in drug and control group compared with sham-operated group and the amount in drug group was higher than in the
control group (P<0.05). The expression levels of VEGF and bFGF in ischemic myocardial drug group and control group were also higher
than sham-operated group (P<0.05) and the highest expression levels were observed in drug group (P<0.05).Conclusion:Notoginsenoside
R1 can promote angiogenesis and increase the expression of VEGF and bFGF in ischemic myocardiumof rats. |
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