| 周莉娜 向江东.N- 三甲基壳聚糖包裹喜树碱治疗卵巢癌淋巴结转移的研究[J].,2014,14(30):5841-5844 |
| N- 三甲基壳聚糖包裹喜树碱治疗卵巢癌淋巴结转移的研究 |
| The Impact on Lymph node Metastasis of Ovarian Cancer Basedon N-trimethyl Chitosan Chloride-encapsulated Camptothecin |
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| DOI： |
| 中文关键词: N- 三甲基壳聚糖 喜树碱 卵巢癌 淋巴结转移 |
| 英文关键词: Camptothecin N-trimethyl chitosan Lymphatic metastasis Ovarian cancer |
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| 中文摘要: |
| 目的：采用N-三甲基壳聚糖包裹喜树碱(CPT-TMC)以增加其水溶性，并检测其抑制卵巢癌细胞淋巴结转移的效应。方法：
体外实验，PI染色检测CPT-TMC 促肿瘤细胞凋亡作用，体内实验，荷瘤裸鼠随机分为4 组（5 只/ 组）：NS 组、TMC 组、CPT 组及
CPT-TMC组，通过尾静脉给药，每周2 次，持续3 周，Evan's Blue Dye 染色，显影腹腔肿大转移的淋巴结。结果：PI染色提示
CPT-TMC 显著促进肿瘤细胞凋亡（P<0.05）；Evan's Blue Dye 显影显示CPT-TMC 显著抑制肿瘤细胞淋巴结转移（P<0.05）。结论：
CPT-TMC具有明显抑制卵巢癌细胞淋巴结转移效应，它可能成为一种新的有效的卵巢癌治疗药物。 |
| 英文摘要: |
| Objective:In the current study, we encapsulated camptothecin with N-trimethyl chitosan (CPT-TMC) to increase its'
solubility and tested it on ovarian cancer model.Methods:For in vitro study, PI staining was performed to evaluate cell apoptosis. For in
vivo study, tumor-bearing mice were randomly divided into four groups (5 per group): NS, TMC, CPT and CPT-TMC group, and
different group received the corresponding treatment via tail vein twice per week for three weeks. Evan's Blue Dye was used to indicate
lymphatic vessels and swell lymph nodes in abdominopelvic cavity.Results:In vitro, PI staining showed CPT-TMC can significantly
induce cell apoptosis (P<0.05). In vivo, CPT-TMC can effectively inhibit metastasis to lymph nodes (P<0.05).Conclusion:The present
findings indicate that CPT-TMC may provide an effective approach to inhibit lymph node metastasis of ovarian cancer and it may be a
potential novel effective approach to ovarian cancer. |
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