| 陆相前 李晓 沈方臻 周玲玲 高鹏.不同剂量的塞来昔布对C57BL/6小鼠移植瘤微淋巴管密度抑制作用的研究[J].,2014,14(29):5629-5632 |
| 不同剂量的塞来昔布对C57BL/6小鼠移植瘤微淋巴管密度抑制作用的研究 |
| The Inhibitory Effect of Different Doses of Celecoxib on LymphaticMicrovessel Density in Tumor Homografts in C57BL/6 Mice |
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| DOI： |
| 中文关键词: 淋巴管的生成 塞来昔布 环氧化酶-2 微淋巴管密度 免疫印迹分析 |
| 英文关键词: Lymphangiogenesis Celecoxib Cyclooxygenase-2 LMVD Western blot |
| 基金项目:国家自然科学基金项目（30872169） |
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| 中文摘要: |
| 目的：观察不同剂量的塞来昔布对C57BL/6 小鼠肺癌移植瘤生长、COX-2 表达和微淋巴管密度影响，探讨塞来昔布对
C57BL/6 小鼠肺癌移植瘤淋巴管生成可能作用机制及量效关系。方法：将Lewis 肺癌细胞株接种于C57BL/6 小鼠左侧腹股沟皮
下建立移植瘤模型，随机分为4 组：对照组、塞来昔布低剂量、中剂量、高剂量组。观察荷瘤小鼠生存状态，瘤体积变化，种瘤42 天
后牺牲小鼠，western blot半定量检测COX-2 表达及微淋巴管密度。结果：Western blot半定量显示：塞来昔布高、中剂量组COX-2
的表达水平及免疫组织化学染色微淋巴管密度计数均明显减低，差异有统计学意义(P＜0.05)，低剂量组略有减低但差异无统计学
意义（P＞0.05）。抑制程度呈明显的剂量依赖性。结论：塞来昔布抑制Lewis肺癌移植瘤的生长及淋巴转移，可能与下调COX-2 的
表达，阻遏了淋巴管生成的信号通路，抑制微淋巴管生成有关，该抑制作用呈一定的剂量相关性。 |
| 英文摘要: |
| Objective:To observe the effects of different doses of celecoxib on the growth of tumor homografts in C57BL/6 mice,
COX-2 expression and lymphatic microvessel density and to explore the possible mechanism of action and dose-effect relationship of
celecoxib on lymphangiogenesis of Lewis lung tumor.Methods:The cell lines of Lewis lung carcinoma were inoculated in the left
inguinal subcutis of C57BL/6 mice for establishment of the tumor homograft model. They were randomly divided into four groups,
including the control group, low-dose, medium-dose and high-dose celecoxib groups. The survival status and tumor volume change were
observed in tumor-bearing mice. The mice were sacrificed 42 days after transplantation of tumors. The tumor tissue was collected for
western blot semi-quantitative detection of COX-2 expression and lymphatic microvessel density (LMVD).Results:Semi-quantitative
western blot determination showed that the expression levels of COX-2 and immunohistochemical-stained lymphatic microvessel density
counting in the high- and medium-dose groups were significantly reduced, and the differences were statistically significant (P<0.05); the
low-dose group was slightly reduced but not significantly different (P>0.05). The degree of inhibition was in a dose-dependent manner.Conclusion:The inhibition of celecoxib on the growth of Lewis lung cancer homografts and lymph node metastasis may be related to the
down-regulation of COX-2 expression, repression of lymphangiogenesis signaling pathways, and inhibition of lymphangiogenesis which
is related to dosage. |
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