| 滕晓丹 张明 李志高 郭欣欣 陈琢 王洪斌.基质金属蛋白酶及其抑制剂在乳腺癌中的表达[J].,2014,14(29):5620-5624 |
| 基质金属蛋白酶及其抑制剂在乳腺癌中的表达 |
| Expression of Tissue Levels of Matrix Metalloproteinases and TheirInhibitors in Breast Cancer |
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| DOI： |
| 中文关键词: 乳腺癌 基质金属蛋白酶 半定量RT-PCR 金属蛋白酶组织抑制剂 |
| 英文关键词: Breast cancer Matrix metalloproteinase Semi-quantitative RT-PCR Tissue inhibitor of metalloproteinase |
| 基金项目:黑龙江省教育厅科学技术研究项目(12531247)；黑龙江省教育厅科学技术研究项目(12531246)；黑龙江省卫生厅科研课题(2013067) |
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| 中文摘要: |
| 目的：研究基质金属蛋白酶2 (Matrix Metalloproteinase-2, MMP-2), 基质金属蛋白酶7（MMP-7），基质金属蛋白酶9
（MMP-9），膜型基质金属蛋白酶(Membrane Type-1 Matrix Metalloproteinase, MT1-MMP)，金属蛋白酶组织抑制剂1(Tissue
Inhibitor of Metalloproteinase, TIMP-1)，金属蛋白酶组织抑制剂2（TIMP-2）在乳腺癌组织中mRNA的表达，及与临床病理变量之
间的关联。方法：采用150 例乳腺癌患者的组织样本。使用半定量逆转录-聚合酶链反应（RT-PCR）法来测定肿瘤组织和正常乳腺
组织中MMP-2，MMP-7，MMP-9，MT1-MMP，TIMP-1 和TIMP-2 的mRNA 表达。结果：MMP-2，MMP-7，MMP-9，MT1-MMP，
TIMP-1 和TIMP-2 在乳腺癌中的mRNA 表达显著高于正常组织。结论：MMP-2，MMP-7，MMP-9，和MTI-MMP的表达增加和临
床病理参数之间的关联，可以用来预测乳腺癌的侵害行为。 |
| 英文摘要: |
| Objective:To investigate mRNA expression of MMP-2, MMP-7, MMP-9, MT1-MMP, TIMP-1, and TIMP-2 in
human breast cancer tissues, and the relationship between their expression and clinicopathological variability.Methods:Breast tissue
samples from 150 patients with breast cancer were available in this study. Semiquantitative reverse transcriptase-polymerase chain
reaction (RT-PCR) was carried out on tumor and normal tissues to determine mRNA expression for MMP-2, MMP-7, MMP-9,
MT1-MMP, TIMP-1, and TIMP-2.Results:The expression of MMP-2, MMP-7, MMP-9, MT1-MMP, TIMP-1, and TIMP-2 mRNA in
the breast cancer was significantly higher than that in the normal tissue. In terms of tumor size and lymph node metastasis of breast
cancer, the differences in MMP-2, MMP-7, MMP-9, and MT1-MMP mRNA expression levels were significant.Conclusion:The
relationship between the increased expression of MMP-2, MMP-7, MMP-9, and MTI-MMP and clinicopathological parameters reflects a
role in predicting the aggressive behavior of breast cancer. |
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