| 申宝玺 陈静 梁文学 陈慧珍 李爱民.PP2 对大鼠面神经缺血损伤的保护作用[J].,2014,14(29):5617-5619 |
| PP2 对大鼠面神经缺血损伤的保护作用 |
| The Protective Effect of PP2 on Ischemic Injury of Facial Nervein a Rat Model |
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| DOI： |
| 中文关键词: 面神经 缺血损伤 NMDA受体 PSD95 Src 激酶 PP2 |
| 英文关键词: Facial nerve Ischemia NMDA receptor PSD95 Src PP2 |
| 基金项目:国家自然科学基金项目（81202610）；江苏省卫生厅重大项目（H201259）；江苏省333人才工程（BRA2010050） |
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| 中文摘要: |
| 目的：探讨PP2（4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine）在大鼠面神经缺血损伤模型中的保护作用，
为进一步探索面神经缺血损伤的治疗方法提供实验依据。方法：实验选取40 只雄性SD大鼠，分为四组：假手术组，损伤组，
DMSO溶剂对照组和PP2 干预组。手术组沿耳廓外上缘行弧形切口，分离暴露出岩动脉，双极电凝灼断。假手术组暴露出岩动脉
但不予灼断。PP2 干预组和DMSO 溶剂对照组于术前30 min分别脑室注射15 滋g(总体积10 滋L)PP2 或相同体积DMSO。于3 d
后观察各组大鼠的行为学变化，同时运用免疫共沉淀和免疫印迹的技术分析其中分子机制。结果：（1）PP2 干预组的大鼠相对于手
术组和DMSO 溶剂对照组，瞬目反射和触须动度明显好转（2）大鼠面神经缺血损伤时，损伤组相对于假手术组，
NMDAR-PSD95-Src 的结合明显增加（P<0.05）（3）PP2 干预组NR2A-PSD95-Src 的结合相对于手术组和DMSO 溶剂对照组明显
降低（P<0.05）。结论：大鼠面神经缺血损伤时，存在NMDAR-PSD95-Src 形成的信号通路，PP2 可以有效的抑制NMDARPSD95-
Src信号通路的形成，从而起到面神经保护作用。 |
| 英文摘要: |
| Objective:To investigate the protective effect of PP2 on the facial nerve ischemia injury in a rat model and to furnish
the theoretic foundation and experimental evidence for further researching on the facial nerve ischemia injury.Methods:40 male SD rats
were selected and divided into four groups: sham group, Injury group, DMSO group, and PP2 group. All animals were anesthetized. The
petrosal artery was interrupted by the bipolar electrocoagulation. The same operation procedures were taken without interrupting the
petrosal artery in the sham group. 15 滋g PP2 (total volume 10 滋L ) were infused into the rat left cereboventricle by a stepper-motorized
microsyringe in the PP2 group and the same dose of DMSO was infused into the rat left cereboventricle in DMSO group. Behavioral
changes were observed after 3 days and the techniques of co-immunoprecipitation and immunoblotting were used to analysis the
Molecular mechanism.Results:(1) Blink reflex and whisker movement of rats in the PP2 group improved significantly. (2) The
interactions of NMDAR with PSD95 and Src increased immediately in the injury group (P<0.05). (3) The increased interactions of
NMDAR with PSD95 and Src were simultaneously suppressed in PP2 group compared with injury and DMSO group.Conclusion:There
is a signal pathway assembled by NMDAR-PSD95-Src in the rat model of facial nerve ischemia. PP2 can inhibit the formation of
NMDAR-PSD95-Src signal pathway effectively and play the role of protection in the rat model of facial nerve ischemia . |
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